PUBLICATION

Sex-specific effects of difenoconazole on the growth hormone endocrine axis in adult zebrafish (Danio rerio)

Authors
Teng, M., Qi, S., Zhu, W., Wang, Y., Wang, D., Yang, Y., Li, H., Li, C., Dong, K., Wang, C.
ID
ZDB-PUB-170627-1
Date
2017
Source
Ecotoxicology and environmental safety   144: 402-408 (Journal)
Registered Authors
Keywords
Difenoconazole, GH/IGF axis, Growth endocrine, Sex-specific, Zebrafish
MeSH Terms
  • Animals
  • Dioxolanes/metabolism
  • Dioxolanes/toxicity*
  • Endocrine Disruptors/metabolism
  • Endocrine Disruptors/toxicity*
  • Female
  • Growth Hormone/metabolism*
  • Liver/drug effects
  • Male
  • Ovary/drug effects
  • Sex Characteristics*
  • Testis/drug effects
  • Triazoles/metabolism
  • Triazoles/toxicity*
  • Water Pollutants, Chemical/metabolism
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/metabolism*
PubMed
28651189 Full text @ Ecotoxicol. Environ. Saf.
Abstract
Difenoconazole, as one of the most widely used triazole fungicides, is applied to protect crops, fruits, and vegetables. It has been reported that difenoconazole can enter the environment and impair aquatic organisms, but whether difenoconazole can disrupt the growth hormone (GH) balance in adult zebrafish (Danio rerio) is still unclear. In this study, adult female and male zebrafish were exposed to difenoconazole (0, 5, 50, and 500µg/L) for seven days. The results revealed that the bioaccumulation of difenoconazole and its primary metabolite difenoconazole alcohol in females were both larger than that in males. In females, the growth of the liver and ovary were inhibited, which may be due to the decreased transcription of the key genes igf1a, igf2a, and igf2b in both organs. Male fish growth was promoted in response to the increased expression of genes relevant to the GH/insulin-like growth factor axis (GH/IGF) axis in the brain, liver, and testis as well as increased GH levels. It was found that difenoconazole interfered with the growth endocrine system and sex-specifically altered the expression of GH/IGF axis related genes in adult zebrafish after a short-term exposure.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping