PUBLICATION
Isolation and Characterization of Isofraxidin 7-O-(6'-O-p-Coumaroyl)-β-glucopyranoside from Artemisia capillaris Thunberg: A Novel, Nontoxic Hyperpigmentation Agent That Is Effective In Vivo.
- Authors
- Yim, S.H., Tabassum, N., Kim, W.H., Cho, H., Lee, J.H., Batkhuu, G.J., Kim, H.J., Oh, W.K., Jung, D.W., Williams, D.R.
- ID
- ZDB-PUB-170621-7
- Date
- 2017
- Source
- Evidence-based complementary and alternative medicine : eCAM 2017: 1401279 (Journal)
- Registered Authors
- Williams, Darren W.
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 28626485 Full text @ Evid. Based Complement. Alternat. Med.
Citation
Yim, S.H., Tabassum, N., Kim, W.H., Cho, H., Lee, J.H., Batkhuu, G.J., Kim, H.J., Oh, W.K., Jung, D.W., Williams, D.R. (2017) Isolation and Characterization of Isofraxidin 7-O-(6'-O-p-Coumaroyl)-β-glucopyranoside from Artemisia capillaris Thunberg: A Novel, Nontoxic Hyperpigmentation Agent That Is Effective In Vivo.. Evidence-based complementary and alternative medicine : eCAM. 2017:1401279.
Abstract
Abnormalities in skin pigmentation can produce disorders such as albinism or melasma. There is a research need to discover novel compounds that safely and effectively regulate pigmentation. To identify novel modulators of pigmentation, we attempted to purify compounds from a bioactive fraction of the Korean medicinal plant Artemisia capillaris Thunberg. The novel compound isofraxidin 7-O-(6'-O-p-coumaroyl)-β-glucopyranoside (compound 1) was isolated and its pigmentation activity was characterized in mammalian melanocytes. Compound 1 stimulated melanin accumulation and increased tyrosinase activity, which regulates melanin synthesis. Moreover, compound 1 increased the expression of tyrosinase and the key melanogenesis regulator microphthalmia-associated transcription factor (MITF) in melanocytes. Compared to the parent compound, isofraxidin, compound 1 produced greater effects on these pigmentation parameters. To validate compound 1 as a novel hyperpigmentation agent in vivo, we utilized the zebrafish vertebrate model. Zebrafish treated with compound 1 showed higher melanogenesis and increased tyrosinase activity. Compound 1 treated embryos had no developmental defects and displayed normal cardiac function, indicating that this compound enhanced pigmentation without producing toxicity. In summary, our results describe the characterization of novel natural product compound 1 and its bioactivity as a pigmentation enhancer, demonstrating its potential as a therapeutic to treat hypopigmentation disorders.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping