PUBLICATION

Adaptive optics improves multiphoton super-resolution imaging

Authors
Zheng, W., Wu, Y., Winter, P., Fischer, R., Nogare, D.D., Hong, A., McCormick, C., Christensen, R., Dempsey, W.P., Arnold, D.B., Zimmerberg, J., Chitnis, A., Sellers, J., Waterman, C., Shroff, H.
ID
ZDB-PUB-170621-2
Date
2017
Source
Nature Methods   14(9): 869-872 (Journal)
Registered Authors
Chitnis, Ajay, Dempsey, William
Keywords
Optical imaging, Super-resolution microscopy
MeSH Terms
  • Equipment Failure Analysis
  • Reproducibility of Results
  • Feedback
  • Image Enhancement/instrumentation*
  • Image Enhancement/methods*
  • Lenses*
  • Sensitivity and Specificity
  • Equipment Design
  • Microscopy, Fluorescence, Multiphoton/instrumentation*
  • Microscopy, Fluorescence, Multiphoton/methods*
(all 10)
PubMed
28628128 Full text @ Nat. Methods
Abstract
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ?250 ?m from the coverslip surface.
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    Citation
    Zheng, W., Wu, Y., Winter, P., Fischer, R., Nogare, D.D., Hong, A., McCormick, C., Christensen, R., Dempsey, W.P., Arnold, D.B., Zimmerberg, J., Chitnis, A., Sellers, J., Waterman, C., Shroff, H. (2017) Adaptive optics improves multiphoton super-resolution imaging. Nature Methods. 14(9):869-872.