PUBLICATION

Ocular vessel patterning in zebrafish is indirectly regulated by Hedgehog signaling

Authors
Weiss, O., Kaufman, R., Mishani, E., Inbal, A.
ID
ZDB-PUB-170618-10
Date
2017
Source
The International journal of developmental biology   61: 277-284 (Journal)
Registered Authors
Inbal, Adi
Keywords
zebrafish, ocular vasculature, Hedgehog, VEGF
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Body Patterning
  • Eye/blood supply
  • Eye/embryology*
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism*
  • Lens, Crystalline/blood supply
  • Lens, Crystalline/embryology*
  • Ligands
  • Neovascularization, Physiologic/physiology
  • Organogenesis
  • Phenotype
  • Retinal Vessels/embryology*
  • Signal Transduction
  • Vascular Endothelial Growth Factor A/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
PubMed
28621424 Full text @ Int. J. Dev. Biol.
Abstract
The superficial ocular vasculature of the embryonic zebrafish develops in a highly stereotypic manner and hence provides a convenient model for studying molecular mechanisms that regulate vascular patterning. We have used transgenic zebrafish embryos in which all endothelial cells express enhanced Green Fluorescent Protein and small molecule inhibitors to examine the contribution of two signaling pathways, vascular endothelial growth factor (VEGF) and Hedgehog (Hh) pathways, to the development of the superficial system. We find that most, but not all vessels of the superficial system depend on VEGF signaling for their growth. Hh signaling appears to limit superficial vessel growth over the dorsal eye and is required to promote superficial vessel growth over the ventral eye. These effects of Hh signaling are indirect. Our initial analyses of factors that regulate growth and patterning of superficial ocular vessels suggest that early patterning events in the embryo during organogenesis stages could influence vascular patterning later on. By studying development of specific vascular systems it should be possible to identify new roles for signaling pathways in regulating vascular development.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes