PUBLICATION
Molecular Mechanisms of Developmental Toxicity Induced by Graphene Oxide at Predicted Environmental Concentrations
- Authors
- Zhang, X., Zhou, Q., Zou, W., Hu, X.
- ID
- ZDB-PUB-170615-3
- Date
- 2017
- Source
- Environmental science & technology 51(14): 7861-7871 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Damage*
- Embryo, Nonmammalian
- Environmental Pollutants/toxicity*
- Graphite/toxicity*
- Oxides
- Reactive Oxygen Species
- Zebrafish/embryology
- PubMed
- 28614664 Full text @ Env. Sci. Tech.
Citation
Zhang, X., Zhou, Q., Zou, W., Hu, X. (2017) Molecular Mechanisms of Developmental Toxicity Induced by Graphene Oxide at Predicted Environmental Concentrations. Environmental science & technology. 51(14):7861-7871.
Abstract
Developmental toxicity is a critical issue in nanotoxicity. However, very little is known about the effects of graphene oxide (GO, a widely used carbon material) at predicted environmental concentrations on biological development or the specific molecular mechanisms. The present study established that the development of zebrafish embryos exposed to trace concentrations (1-100 μg/L) of GO was impaired because of DNA modification, protein carbonylation and excessive generation of reactive oxygen species (ROS), especially the superoxide radical. Noticeably, there was a non-monotonic response of zebrafish developmental toxicity to GO at μg/L to mg/L levels. Transcriptomics analysis revealed that disturbing collagen- and matrix metalloproteinase (MMP)-related genes affected the skeletal and cardiac development of zebrafish. Moreover, metabolomics analysis showed that the inhibition of amino acid metabolism and the ratios of unsaturated fatty acids (UFAs) to saturated fatty acids (SFAs) contributed to the above developmental toxicity. The present work verifies the developmental toxicity of GO at trace concentrations and illustrates for the first time the specific molecular mechanisms thereof. Because of the potential developmental toxicity of GO at trace concentrations, government administrators and nanomaterial producers should consider its potential risks prior to the widespread environmental exposure to GO.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping