PUBLICATION
Anti-angiogenic Activity of Water Extract from Euphorbia pekinensis Rupr
- Authors
- Zhang, W., Liu, B., Feng, Y., Liu, J., Ma, Z., Zheng, J., Xia, Q., Ni, Y., Li, F., Lin, R.
- ID
- ZDB-PUB-170613-4
- Date
- 2017
- Source
- Journal of ethnopharmacology 206: 337-346 (Journal)
- Registered Authors
- Keywords
- Euphorbia pekinensis Rupr., HUVEC, anti-angiogenesis, quantitative real-time PCR, transgenic zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Euphorbia/chemistry*
- Human Umbilical Vein Endothelial Cells
- Humans
- Neovascularization, Pathologic/drug therapy*
- Neovascularization, Pathologic/prevention & control
- Plant Extracts/pharmacology
- Plant Extracts/therapeutic use*
- RNA, Messenger/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Water
- Zebrafish/embryology
- PubMed
- 28602865 Full text @ J. Ethnopharmacol.
Citation
Zhang, W., Liu, B., Feng, Y., Liu, J., Ma, Z., Zheng, J., Xia, Q., Ni, Y., Li, F., Lin, R. (2017) Anti-angiogenic Activity of Water Extract from Euphorbia pekinensis Rupr. Journal of ethnopharmacology. 206:337-346.
Abstract
Ethnopharmacological relevance Euphorbia pekinensis Rupr. (EP) is a Euphorbia species of Euphorbiaceae, which is widely used in traditional Chinese medicine. It has been reported to exhibit therapeutic effects on solid tumors, leukemias, and malignant ascites although underlying molecular mechanisms are poorly delineated. Anti-angiogenic therapy is a recognized strategy for treating cancer-based solid tumors, and is also associated with malignant ascites treatment. Study Aim To study the anti-angiogenic properties of the water extract of EP vinegar preparation (WEVEP).
Materials and methods Following WEVEP treatment, intersegmental blood vessels were assessed during the development of transgenic Tg (flk: mCherry) zebrafish as was the proliferation, migration and network formation of HUVECs in vitro. mRNA expression of specific angiogenic-related genes including VEGF family members, Met, and NRP2 was also measured using quantitative real-time PCR (Q-PCR).
Results Data demonstrated that angiogenesis was inhibited by the WEVEP in zebrafish (from 100µg/mL to 250µg/mL, p < 0.0001) and in the HUVEC model (from 100µg/mL to 400µg/mL, p < 0.0001). In the zebrafish model, the mean vessel numbers of administered groups were 26.00 ±1.29 (100µg/mL), 24.54 ± 2.20 (150µg/mL), 22.66 ± 2.68 (200µg/mL), 20.80 ±1.75 (250µg/mL), compared to 27.67±0.96 of control group. Relative quantitative gene expression in zebrafish treated with WEVEP demonstrated that only VEGFR3 was significantly increased and other 23 genes including Met, VEGFA, Flt-1 were significantly decreased.
Conclusion WEVEP can positively modulate angiogenesis via multiple targeting mechanisms. Our novel results contribute towards the discovery of a possible mechanism(s) of the traditional use of EP in the treatment of cancer and malignant ascites.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping