PUBLICATION
Transmission Electron Microscopy of Zebrafish Spinal Motor Nerve Roots
- Authors
- Morris, A.D., Erisir, A., Criswell, S.J., Kucenas, S.
- ID
- ZDB-PUB-170610-5
- Date
- 2017
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 246(11): 956-962 (Journal)
- Registered Authors
- Kucenas, Sarah
- Keywords
- myelin, peripheral nervous system, spinal motor nerve, zebrafish
- MeSH Terms
-
- Animals
- Histological Techniques/methods
- Microscopy, Electron, Transmission/methods*
- Spinal Nerve Roots/ultrastructure*
- Zebrafish/anatomy & histology
- PubMed
- 28598521 Full text @ Dev. Dyn.
Citation
Morris, A.D., Erisir, A., Criswell, S.J., Kucenas, S. (2017) Transmission Electron Microscopy of Zebrafish Spinal Motor Nerve Roots. Developmental Dynamics : an official publication of the American Association of Anatomists. 246(11):956-962.
Abstract
Background Spinal motor nerves are essential for relaying information between the central and peripheral nervous systems. Perturbations to cell types that compose these nerves may impair rapid and efficient transmission of action potentials and alter nerve function. Identifying ultrastructural changes resulting from defects to these cellular components via transmission electron microscopy (TEM) can provide valuable insight into nerve function and disease. However, efficiently locating spinal motor nerves in adult zebrafish for TEM is challenging and time consuming. Because of this, we developed a protocol that allows us to quickly and precisely locate spinal motor nerve roots in adult zebrafish for TEM processing.
Results Following fixation, a transverse slab of adult zebrafish dissected from the trunk region was mounted in embedding media, sectioned and secondary fixation with osmium tetraoxide was performed. Transverse sections containing motor nerves were selected for TEM ultrathin sectioning and imaging.
Conclusions We developed an efficient protocol for locating spinal motor nerves in adult zebrafish to allow for ultrastructural characterization. Although our work focuses on spinal motor nerves, this protocol may be useful for efficiently identifying other discrete, repeated structures within the developing and mature nervous system that are difficult to find via traditional, whole organism TEM processing. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping