PUBLICATION
Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
- Authors
- Puttonen, H.A.J., Semenova, S., Sundvik, M., Panula, P.
- ID
- ZDB-PUB-170610-10
- Date
- 2017
- Source
- Scientific Reports 7: 3060 (Journal)
- Registered Authors
- Panula, Pertti, Puttonen, Henri, Semenova, Svetlana, Sundvik, Maria
- Keywords
- Cellular neuroscience, Diseases of the nervous system
- MeSH Terms
-
- Animals
- Antipsychotic Agents/pharmacology
- Brain/cytology
- Brain/metabolism
- Brain/physiology
- Histamine/metabolism*
- Neurons/drug effects
- Neurons/metabolism
- Reserpine/pharmacology
- Synaptic Transmission*
- Vesicular Monoamine Transport Proteins/antagonists & inhibitors
- Vesicular Monoamine Transport Proteins/metabolism*
- Zebrafish
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/metabolism*
- PubMed
- 28596586 Full text @ Sci. Rep.
Citation
Puttonen, H.A.J., Semenova, S., Sundvik, M., Panula, P. (2017) Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish. Scientific Reports. 7:3060.
Abstract
Monoaminergic neurotransmission is greatly dependent on the function of the vesicular monoamine transporter VMAT2, which is responsible for loading monoamines into secretory vesicles. The role of VMAT2 in histaminergic neurotransmission is poorly understood. We studied the structure and function of the histaminergic system in larval zebrafish following inhibition of VMAT2 function by reserpine. We found that reserpine treatment greatly reduced histamine immunoreactivity in neurons and an almost total disappearance of histamine-containing nerve fibers in the dorsal telencephalon and habenula, the most densely innervated targets of the hypothalamic histamine neurons. The reserpine treated larvae had an impaired histamine-dependent dark-induced flash response seen during the first second after onset of darkness, implying that function of the histaminergic network is VMAT2 dependent. Levels of histamine and other monoamines were decreased in reserpine treated animals. This study provides conclusive evidence of the relevance of VMAT2 in histaminergic neurotransmission, further implying that the storage and release mechanism of neural histamine is comparable to that of other monoamines. Our results also reveal potential new insights about the roles of monoaminergic neurotransmitters in the regulation of locomotion increase during adaptation to darkness.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping