PUBLICATION
Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish
- Authors
- Shitasako, S., Ito, Y., Ito, R., Ueda, Y., Shimizu, Y., Ohshima, T.
- ID
- ZDB-PUB-170608-7
- Date
- 2017
- Source
- Developmental Neurobiology 77(10): 1206-1220 (Journal)
- Registered Authors
- Ito, Ryoichi, Ohshima, Toshio, Shimizu, Yuki, Ueda, Yuto
- Keywords
- none
- MeSH Terms
-
- Superior Colliculi/drug effects
- Superior Colliculi/metabolism*
- Immunohistochemistry
- Hedgehog Proteins/antagonists & inhibitors
- Hedgehog Proteins/metabolism
- Thiadiazoles/pharmacology
- SOX Transcription Factors/metabolism
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/metabolism*
- Microscopy, Confocal
- Bromodeoxyuridine
- Animals, Genetically Modified
- Quinolines/pharmacology
- Animals
- Cell Proliferation/drug effects
- Cell Proliferation/physiology*
- Patched-1 Receptor/metabolism
- Microscopy, Fluorescence
- Proliferating Cell Nuclear Antigen/metabolism
- Central Nervous System Agents/pharmacology
- Neurogenesis/drug effects
- Neurogenesis/physiology*
- Zebrafish
- Neural Stem Cells/drug effects
- Neural Stem Cells/physiology*
- Imides/pharmacology
- Wnt Signaling Pathway/drug effects
- Wnt Signaling Pathway/genetics
- Wnt Signaling Pathway/physiology
- Patched-2 Receptor/metabolism
- PubMed
- 28589698 Full text @ Dev. Neurobiol.
Citation
Shitasako, S., Ito, Y., Ito, R., Ueda, Y., Shimizu, Y., Ohshima, T. (2017) Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish. Developmental Neurobiology. 77(10):1206-1220.
Abstract
Adult neurogenesis occurs more commonly in teleosts, represented by zebrafish, than in mammals. Zebrafish is therefore considered a suitable model to study adult neurogenesis, for which the regulatory molecular mechanisms remain little known. Our previous study revealed that neuroepithelial-like neural stem cells (NSCs) are located at the edge of the dorsomedial region. We also showed that Notch signaling inhibits NSC proliferation in this region. In the present study, we reported the expression of Wnt and Shh signaling components in this region of the optic tectum. Moreover, inhibitors of Wnt and Shh signaling suppressed NSC proliferation, suggesting that these pathways promote NSC proliferation. Shh is particularly required for maintaining Sox2-positive NSCs. Our experimental data also indicate the involvement of these signaling pathways in neural differentiation from NSCs. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping