Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue
- Zhou, S.R., Guo, L., Wang, X., Liu, Y., Peng, W.Q., Liu, Y., Wei, X.B., Dou, X., Ding, M., Lei, Q.Y., Qian, S.W., Li, X., Tang, Q.Q.
- Molecular and cellular biology 37(16): e00058-17 (Journal)
- Registered Authors
- Wang, Xu
- WAT, cavin-1, acetylation, HSL, lipolysis
- MeSH Terms
- 3T3-L1 Cells
- Acetylation/drug effects
- Adipose Tissue, White/drug effects
- Adipose Tissue, White/metabolism*
- Amino Acid Sequence
- Cell Membrane/drug effects
- Cell Membrane/metabolism
- HEK293 Cells
- Lipolysis*/drug effects
- Membrane Proteins/chemistry
- Membrane Proteins/metabolism*
- Mice, Inbred C57BL
- Protein Binding/drug effects
- RNA-Binding Proteins/chemistry
- RNA-Binding Proteins/metabolism*
- Sirtuin 1/metabolism
- p300-CBP Transcription Factors/metabolism
- 28559430 Full text @ Mol. Cell. Biol.
Zhou, S.R., Guo, L., Wang, X., Liu, Y., Peng, W.Q., Liu, Y., Wei, X.B., Dou, X., Ding, M., Lei, Q.Y., Qian, S.W., Li, X., Tang, Q.Q. (2017) Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue. Molecular and cellular biology. 37(16):e00058-17.
White adipose tissue (WAT) serves as a reversible energy storage depot in the form of lipids in response to nutritional status. Cavin-1, an essential component in the biogenesis of caveolae, is a positive regulator of lipolysis in adipocytes. However, molecular mechanisms of cavin-1 in the modulation of lipolysis remain poorly understood. Here, we showed that cavin-1 was acetylated at lysines 291, 293, and 298 (3K), which was under nutritional regulation in WAT. We further identified GCN5 as the acetyltransferase and Sirt1 as the deacetylase of cavin-1. Acetylation-mimetic 3Q mutants of cavin-1 augmented fat mobilization in 3T3-L1 adipocytes and zebrafish. Mechanistically, acetylated cavin-1 preferentially interacted with hormone-sensitive lipase and recruited it to the caveolae, thereby promoting lipolysis. Our finding shed light on essential role of cavin-1 in regulating lipolysis via an acetylation-dependent manner in WAT.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes