PUBLICATION

Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue

Authors
Zhou, S.R., Guo, L., Wang, X., Liu, Y., Peng, W.Q., Liu, Y., Wei, X.B., Dou, X., Ding, M., Lei, Q.Y., Qian, S.W., Li, X., Tang, Q.Q.
ID
ZDB-PUB-170601-5
Date
2017
Source
Molecular and cellular biology   37(16): e00058-17 (Journal)
Registered Authors
Wang, Xu
Keywords
WAT, cavin-1, acetylation, HSL, lipolysis
MeSH Terms
  • 3T3-L1 Cells
  • Acetylation/drug effects
  • Adipose Tissue, White/drug effects
  • Adipose Tissue, White/metabolism*
  • Amino Acid Sequence
  • Animals
  • Cell Membrane/drug effects
  • Cell Membrane/metabolism
  • HEK293 Cells
  • Humans
  • Lipase/metabolism
  • Lipolysis*/drug effects
  • Lysine/metabolism
  • Male
  • Membrane Proteins/chemistry
  • Membrane Proteins/metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Niacinamide/pharmacology
  • Protein Binding/drug effects
  • RNA-Binding Proteins/chemistry
  • RNA-Binding Proteins/metabolism*
  • Sirtuin 1/metabolism
  • Zebrafish
  • p300-CBP Transcription Factors/metabolism
PubMed
28559430 Full text @ Mol. Cell. Biol.
Abstract
White adipose tissue (WAT) serves as a reversible energy storage depot in the form of lipids in response to nutritional status. Cavin-1, an essential component in the biogenesis of caveolae, is a positive regulator of lipolysis in adipocytes. However, molecular mechanisms of cavin-1 in the modulation of lipolysis remain poorly understood. Here, we showed that cavin-1 was acetylated at lysines 291, 293, and 298 (3K), which was under nutritional regulation in WAT. We further identified GCN5 as the acetyltransferase and Sirt1 as the deacetylase of cavin-1. Acetylation-mimetic 3Q mutants of cavin-1 augmented fat mobilization in 3T3-L1 adipocytes and zebrafish. Mechanistically, acetylated cavin-1 preferentially interacted with hormone-sensitive lipase and recruited it to the caveolae, thereby promoting lipolysis. Our finding shed light on essential role of cavin-1 in regulating lipolysis via an acetylation-dependent manner in WAT.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes