PUBLICATION

Efficient Transduction of Zebrafish Melanoma Cell Lines and Embryos Using Lentiviral Vectors

Authors
Fazio, M., Avagyan, S., van Rooijen, E., Mannherz, W., Kaufman, C.K., Lobbardi, R., Langenau, D.M., Zon, L.I.
ID
ZDB-PUB-170531-3
Date
2017
Source
Zebrafish   14(4): 379-382 (Journal)
Registered Authors
Langenau, David, van Rooijen, Ellen, Zon, Leonard I.
Keywords
gene delivery, lentiviral, lentivirus, retrovirus, viral vectors, zebrafish cell lines
MeSH Terms
  • Animals
  • Genetic Vectors/administration & dosage*
  • Lentivirus/genetics*
  • Melanoma/genetics*
  • Melanoma/pathology
  • Membrane Glycoproteins/genetics
  • Retroviridae/genetics*
  • Transduction, Genetic*
  • Tumor Cells, Cultured
  • Viral Envelope Proteins/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/growth & development
PubMed
28557653 Full text @ Zebrafish
Abstract
The establishment of in vitro cultures of zebrafish cancer cells has expanded the potential of zebrafish as a disease model. However, the lack of effective methods for gene delivery and genetic manipulation has limited the experimental applications of these cultures. To overcome this barrier, we tested and optimized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviral and retroviral vector transduction protocols. We show that lentivirus successfully and efficiently transduced zebrafish melanoma cell lines in vitro, allowing antibiotic selection, fluorescence-based sorting, and in vivo allotransplantation. In addition, injection of concentrated lentiviral particles into embryos and tumors established the feasibility of in vivo gene delivery.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes