PUBLICATION

Manganese(II) Chloride Alters Nucleotide and Nucleoside Catabolism in Zebrafish (Danio rerio) Adult Brain

Authors
Altenhofen, S., Nabinger, D.D., Pereira, T.C.B., Leite, C.E., Bogo, M.R., Bonan, C.D.
ID
ZDB-PUB-170527-4
Date
2017
Source
Molecular neurobiology   55(5): 3866-3874 (Journal)
Registered Authors
Bonan, Carla Denise
Keywords
Adenosine deaminase, Manganese(II) chloride, NTPDases, Purinergic signaling, Zebrafish
MeSH Terms
  • Adenosine Deaminase/metabolism
  • Aging/metabolism*
  • Animals
  • Antigens, CD
  • Apyrase
  • Brain/metabolism*
  • Chlorides/pharmacology*
  • Female
  • Male
  • Manganese Compounds/pharmacology*
  • Nucleosides/metabolism*
  • Nucleotides/metabolism*
  • Zebrafish/metabolism*
PubMed
28547528 Full text @ Mol. Neurobiol.
Abstract
ATP and adenosine, the main signaling molecules of purinergic system, are involved in toxicological effects induced by metals. The manganese (Mn) exposure induces several cellular changes, which could interfere with signaling pathways, such as the purinergic system. In this study, we evaluated the effects of exposure to manganese(II) chloride (MnCl2) during 96 h on nucleoside triphosphate diphosphohydrolase (NTPDase), ecto-5'-nucleotidase, and adenosine deaminase (ADA) activities, followed by analyzing the gene expression patterns of NTPDases (entpd1, entpd2a.1, entpd2a.2, entpd2-like, entpd3) and ADA (ADA 1 , ADA 2.1 , ADA 2.2 , ADAasi, ADAL) families in zebrafish brain. In addition, the brain metabolism of nucleotides and nucleosides was evaluated after MnCl2 exposure. The results showed that MnCl2 exposure during 96 h inhibited the NTPDase (1.0 and 1.5 mM) and ecto-ADA (0.5, 1.0, and 1.5 mM) activities, further decreasing ADA2.1 expression at all MnCl2 concentrations analyzed. Purine metabolism was also altered by the action of MnCl2. An increased amount of ADP appeared at all MnCl2 concentrations analyzed; however, AMP and adenosine levels are decreased at the concentrations of 1.0 and 1.5 mM MnCl2, whereas decreased inosine (INO) levels were observed at all concentrations tested. The findings of this study demonstrated that MnCl2 may inhibit NTPDase and ecto-ADA activities, consequently modulating nucleotide and nucleoside levels, which may contribute for the toxicological effects induced by this metal.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping