PUBLICATION

Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation

Authors
Krens, S.F.G., Veldhuis, J.H., Barone, V., ?apek, D., Maître, J.L., Brodland, G.W., Heisenberg, C.P.
ID
ZDB-PUB-170518-6
Date
2017
Source
Development (Cambridge, England)   144: 1798-1806 (Journal)
Registered Authors
Barone, Vanessa, Heisenberg, Carl-Philipp, Krens, S. F. Gabby, Maître, Jean-Léon
Keywords
Cell internalization, Gastrulation, Tissue surface tension, Zebrafish
MeSH Terms
  • Mesoderm/chemistry
  • Mesoderm/cytology
  • Mesoderm/embryology
  • Embryo, Nonmammalian
  • Stem Cells/chemistry*
  • Stem Cells/cytology
  • Stem Cells/physiology*
  • Cell Movement*
  • Osmolar Concentration
  • Body Patterning*
  • Zebrafish/embryology*
  • Animals
  • Extracellular Fluid/chemistry*
  • Gastrulation/physiology*
  • Surface Tension
  • Animals, Genetically Modified
(all 16)
PubMed
28512197 Full text @ Development
Abstract
The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e100TgTransgenic Insertion
    e114TgTransgenic Insertion
      e119TgTransgenic Insertion
        e1954TgTransgenic Insertion
          e2212TgTransgenic Insertion
            ml1TgTransgenic Insertion
              tz257
                		Point Mutation
                vu119TgTransgenic Insertion
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                  Target Reagent Reagent Type
                  sox32MO1-sox32MRPHLNO
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                  Marker Marker Type Name
                  EGFPEFGEGFP
                  GFPEFGGFP
                  mCherryEFGmCherry
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                  Citation
                  Krens, S.F.G., Veldhuis, J.H., Barone, V., ?apek, D., Maître, J.L., Brodland, G.W., Heisenberg, C.P. (2017) Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development (Cambridge, England). 144:1798-1806.