PUBLICATION
A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
- Authors
- Lee, J., Chou, T.C., Kang, D., Kang, H., Chen, J., Baek, K.I., Wang, W., Ding, Y., Carlo, D.D., Tai, Y.C., Hsiai, T.K.
- ID
- ZDB-PUB-170518-4
- Date
- 2017
- Source
- Scientific Reports 7: 1980 (Journal)
- Registered Authors
- Keywords
- Animal physiology, Biomedical engineering
- MeSH Terms
-
- Animals
- Biomechanical Phenomena
- Blood Viscosity
- Hemodynamics*
- Hemorheology*
- Microfluidics/methods
- Reproducibility of Results
- Stress, Mechanical
- Zebrafish/physiology*
- PubMed
- 28512313 Full text @ Sci. Rep.
Citation
Lee, J., Chou, T.C., Kang, D., Kang, H., Chen, J., Baek, K.I., Wang, W., Ding, Y., Carlo, D.D., Tai, Y.C., Hsiai, T.K. (2017) A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates. Scientific Reports. 7:1980.
Abstract
Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s-1), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s-1 were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping