PUBLICATION
Lineage tracing of dlx1a/2a and dlx5a/6a expressing cells in the developing zebrafish brain
- Authors
- Solek, C.M., Feng, S., Perin, S., Weinschutz Mendes, H.C., Ekker, M.
- ID
- ZDB-PUB-170510-12
- Date
- 2017
- Source
- Developmental Biology 427(1): 131-147 (Journal)
- Registered Authors
- Ekker, Marc
- Keywords
- Dlx, GABA, lineage tracing, zebrafish
- MeSH Terms
-
- Animals
- Brain/cytology
- Brain/embryology
- Brain/metabolism*
- Cell Lineage/genetics
- Embryo, Mammalian/cytology
- Embryo, Mammalian/embryology
- Embryo, Mammalian/metabolism
- GABAergic Neurons/cytology
- GABAergic Neurons/metabolism*
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Immunohistochemistry
- In Situ Hybridization
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Mice
- Mice, Transgenic
- Microscopy, Confocal
- Microscopy, Fluorescence
- Mouse Embryonic Stem Cells/cytology
- Mouse Embryonic Stem Cells/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 28479339 Full text @ Dev. Biol.
Citation
Solek, C.M., Feng, S., Perin, S., Weinschutz Mendes, H.C., Ekker, M. (2017) Lineage tracing of dlx1a/2a and dlx5a/6a expressing cells in the developing zebrafish brain. Developmental Biology. 427(1):131-147.
Abstract
Lineage tracing of specific populations of progenitor cells provides crucial information about developmental programs. Four members of the Dlx homeobox gene family, Dlx1,2, 5 and 6, are involved in the specification of γ-aminobutyric acid (GABA)ergic neurons in the vertebrate forebrain. Orthologous genes in mammals and teleost show similarities in expression patterns and transcriptional regulation mechanisms. We have used lineage tracing to permanently label dlx-expressing cells in the zebrafish and have characterized the progeny of these cells in the larva and in the juvenile and adult brain. We have found that dlx1a/2a and dlx5a/6a expressing progenitors give rise, for the most part, to small populations of cells which constitute only a small proportion of GABAergic cells in the adult brain tissue. Moreover, some of the cells do not acquire a neuronal phenotype suggesting that, regardless of the time a cell expresses dlx genes in the brain, it can potentially give rise to cells other than neurons. In some instances, labeling larval dlx5a/6a-expressing cells, but not dlx1a/2a-expressing cells, results in massively expanding, widespread clonal expansion throughout the adult brain. Our data provide a detailed lineage analysis of the dlx1a/2a and dlx5a/6a expressing progenitors in the zebrafish brain and lays the foundation for further characterization of the role of these transcription factors beyond the specification of GABAergic neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping