PUBLICATION
Whole extracts of Radix Achyranthis Bidentatae and Radix Cyathulae promote angiogenesis in human umbilical vein endothelial cells in vitro and in zebrafish in vivo.
- Authors
- Zhou, X., Siu, W.S., Zhang, C., Liu, C.L., Cheng, L., Kwok, H.F., Fung, C.H., Tam, J.C., Lau, C.P., Lau, C.B., Leung, P.C., Hung, L.K., Ko, C.H.
- ID
- ZDB-PUB-170430-5
- Date
- 2017
- Source
- Experimental and Therapeutic Medicine 13: 1032-1038 (Journal)
- Registered Authors
- Keywords
- Radix Achyranthis Bidentatae, cell migration, pro-angiogenesis, radix cyathulae, zebrafish
- MeSH Terms
- none
- PubMed
- 28450937 Full text @ Exp. Ther. Med.
Citation
Zhou, X., Siu, W.S., Zhang, C., Liu, C.L., Cheng, L., Kwok, H.F., Fung, C.H., Tam, J.C., Lau, C.P., Lau, C.B., Leung, P.C., Hung, L.K., Ko, C.H. (2017) Whole extracts of Radix Achyranthis Bidentatae and Radix Cyathulae promote angiogenesis in human umbilical vein endothelial cells in vitro and in zebrafish in vivo.. Experimental and Therapeutic Medicine. 13:1032-1038.
Abstract
Although Radix Achyranthis Bidentatae (RAB) and Radix Cyathulae (RC) are from two different medicinal plants, they are both used as 'Niu-Xi', a widely used traditional Chinese medicine that is believed to stimulate menstruation and affect bone injury. Angiogenesis is actively involved in treating these illnesses. The aim of the present study was to investigate whether the whole extracts of RAB and RC possess pro-angiogenic effects. In order to examine this idea whole extracts of RAB and RC were extracted with boiling water followed by ethanol, respectively. Results from the MTT, wound healing and tube formation assays in human umbilical vein endothelial cells (HUVECs) in vitro revealed that the whole extracts of RAB and RC did not increase cell proliferation or tube formation, but enhanced cell migration. Their angiogenic effects were also confirmed in zebrafish in vivo via increasing the sprout numbers in the sub-intestinal vessel. As determined by quantitative polymerase chain reaction, the whole extracts of RAB and RC both regulated the expression of cell migration-related genes in zebrafish. It is concluded that the whole extracts of RAB and RC induced angiogenesis in HUVECs in vitro and in zebrafish in vivo via increasing cell migration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping