PUBLICATION
Tebuconazole alters morphological, behavioral and neurochemical parameters in larvae and adult zebrafish (Danio rerio)
- Authors
- Altenhofen, S., Nabinger, D.D., Wiprich, M.T., Pereira, T.C.B., Bogo, M.R., Bonan, C.D.
- ID
- ZDB-PUB-170422-1
- Date
- 2017
- Source
- Chemosphere 180: 483-490 (Journal)
- Registered Authors
- Bonan, Carla Denise
- Keywords
- Acetylcholine, Acetylcholinesterase, Behavior, Morphological parameters, Tebuconazole, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Brain/drug effects
- Exploratory Behavior
- Fungicides, Industrial/metabolism
- Fungicides, Industrial/toxicity*
- Larva/drug effects
- Locomotion/drug effects
- Triazoles/toxicity*
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism
- Zebrafish/physiology*
- PubMed
- 28431386 Full text @ Chemosphere
Citation
Altenhofen, S., Nabinger, D.D., Wiprich, M.T., Pereira, T.C.B., Bogo, M.R., Bonan, C.D. (2017) Tebuconazole alters morphological, behavioral and neurochemical parameters in larvae and adult zebrafish (Danio rerio). Chemosphere. 180:483-490.
Abstract
In this study, we evaluated the effects of tebuconazole on morphology and exploratory larvae behavior and adult locomotion. Furthermore, we analyzed the effects of this fungicide on AChE activity and gene expression in zebrafish larvae and in the adult zebrafish brain. Tebuconazole (4 mg/L) increased the ocular distance in larvae and reduced the distance travelled, absolute turn angle, line crossing and time outside area in exposed larvae. Moreover, adult zebrafish that were exposed to this fungicide (4 and 6 mg/L) showed a decrease in distance travelled and mean speed when compared to the control group. However, tebuconazole did not alter the number of line crossings or time spent in the upper zone. Tebuconazole inhibited AChE activity at concentrations of 4 mg/L for larvae and 4 and 6 mg/L in the adult zebrafish brain. However, this fungicide did not alter AChE gene expression in the adult zebrafish brain but increased AChE mRNA transcript levels in larvae. These findings demonstrated that tebuconazole could modulate the cholinergic system by altering AChE activity and that this change may be associated with the reduced locomotion of these animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping