PUBLICATION

The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes

Authors
Djenoune, L., Desban, L., Gomez, J., Sternberg, J.R., Prendergast, A., Langui, D., Quan, F.B., Marnas, H., Auer, T.O., Rio, J.P., Del Bene, F., Bardet, P.L., Wyart, C.
ID
ZDB-PUB-170409-2
Date
2017
Source
Scientific Reports   7: 719 (Journal)
Registered Authors
Auer, Thomas, Bardet, Pierre-Luc, Del Bene, Filippo, Djenoune, Lydia, Prendergast, Andrew, Wyart, Claire
Keywords
Cellular neuroscience, Neurophysiology
MeSH Terms
  • Spinal Nerve Roots
  • Biomarkers
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Cerebrospinal Fluid/metabolism*
  • Fluorescent Antibody Technique
  • Mutation
  • Homozygote
  • Spinal Cord/cytology*
  • Spinal Cord/physiology*
  • Signal Transduction*
  • Animals
  • Neurons/physiology*
  • Neurons/ultrastructure
  • Ganglia, Spinal
  • Cell Differentiation
  • Sensory Receptor Cells/physiology
  • Sensory Receptor Cells/ultrastructure
  • Zebrafish
  • Animals, Genetically Modified
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Axons/physiology
  • Axons/ultrastructure
(all 24)
PubMed
28389647 Full text @ Sci. Rep.
Abstract
Chemical and mechanical cues from the cerebrospinal fluid (CSF) can affect the development and function of the central nervous system (CNS). How such cues are detected and relayed to the CNS remains elusive. Cerebrospinal fluid-contacting neurons (CSF-cNs) situated at the interface between the CSF and the CNS are ideally located to convey such information to local networks. In the spinal cord, these GABAergic neurons expressing the PKD2L1 channel extend an apical extension into the CSF and an ascending axon in the spinal cord. In zebrafish and mouse spinal CSF-cNs originate from two distinct progenitor domains characterized by distinct cascades of transcription factors. Here we ask whether these neurons with different developmental origins differentiate into cells types with different functional properties. We show in zebrafish larva that the expression of specific markers, the morphology of the apical extension and axonal projections, as well as the neuronal targets contacted by CSF-cN axons, distinguish the two CSF-cN subtypes. Altogether our study demonstrates that the developmental origins of spinal CSF-cNs give rise to two distinct functional populations of sensory neurons. This work opens novel avenues to understand how these subtypes may carry distinct functions related to development of the spinal cord, locomotion and posture.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
nns14Tgstandard conditionsProtruding-mouthIHC
uaa6Tgstandard conditionsProtruding-mouthIHC
icm07Tgstandard conditionsLong-pecIHC
icm07Tgstandard conditionsPrim-5IHC
icm07Tgstandard conditionsProtruding-mouthIHC
icm07Tgstandard conditionsPrim-5IHC
icm07Tgstandard conditionsLong-pecIHC
icm07Tgstandard conditionsProtruding-mouthIHC
icm07Tgstandard conditionsPrim-5IHC
icm07Tgstandard conditionsProtruding-mouthIHC
1 - 10 of 20
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
icm02
    		Small Deletion
    icm07TgTransgenic Insertion
      icm10TgTransgenic Insertion
        icm22TgTransgenic Insertion
          icm28TgTransgenic Insertion
            ml2TgTransgenic Insertion
              mn2EtTransgenic Insertion
              nns14TgTransgenic Insertion
                uaa6TgTransgenic Insertion
                  1 - 9 of 9
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  Fish
                  icm07Tg
                  icm10Tg; icm22Tg
                  ml2Tg
                  nns14Tg
                  pargamn2Et
                  uaa6Tg
                  1 - 6 of 6
                  Show
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  DsRedEFGDsRed
                  EGFPEFGEGFP
                  GAL4EFGGAL4
                  GCaMPEFGGCaMP
                  GFPEFGGFP
                  TagRFPEFGTagRFP
                  VenusEFGVenus
                  1 - 7 of 7
                  Show
                  Mapping
                  No data available
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                  Citation
                  Djenoune, L., Desban, L., Gomez, J., Sternberg, J.R., Prendergast, A., Langui, D., Quan, F.B., Marnas, H., Auer, T.O., Rio, J.P., Del Bene, F., Bardet, P.L., Wyart, C. (2017) The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes. Scientific Reports. 7:719.