PUBLICATION
Retinoblastoma binding protein 6 and crystallin lambda 1 are cadmium-responsive genes in zebrafish embryos and adults retinae
- Authors
- Scudiero, R., Esposito, M.G., Simoniello, P., Motta, C.M.
- ID
- ZDB-PUB-170408-9
- Date
- 2017
- Source
- Comptes rendus biologies 340(4): 197-203 (Journal)
- Registered Authors
- Keywords
- Adult zebrafish retina, Cadmium, Gene expression, In situ hybridization, Zebrafish embryos, mRNA differential display
- MeSH Terms
-
- Animals
- Cadmium/toxicity*
- Crystallins/biosynthesis
- Crystallins/genetics*
- Embryo, Nonmammalian/drug effects
- Female
- Gene Expression/drug effects
- Male
- Retina/drug effects*
- Retina/physiology
- Retinoblastoma Binding Proteins/biosynthesis
- Retinoblastoma Binding Proteins/genetics*
- Zebrafish
- PubMed
- 28385620 Full text @ C. R. Biol.
Citation
Scudiero, R., Esposito, M.G., Simoniello, P., Motta, C.M. (2017) Retinoblastoma binding protein 6 and crystallin lambda 1 are cadmium-responsive genes in zebrafish embryos and adults retinae. Comptes rendus biologies. 340(4):197-203.
Abstract
Nonessential metal cadmium is widely used and released in the environment, causing cell toxicity and posing a severe threat to wildlife. Zebrafish (Danio rerio) is one of the most commonly used animals in the investigation of environmental cadmium toxicity in vertebrates. In this study, we identified two cadmium-responsive genes, RBBP6 and CRYL1, in the early phases of zebrafish development, at the gastrula stage. The retinoblastoma binding protein 6 is associated with increased protein degradation and cell proliferation; crystallin-lambda 1 is a lens protein with redox activity. In situ hybridization analysis performed on adult zebrafish exposed to 1.5-40 μM cadmium for 30 days confirmed the ability of cadmium to up-regulate the expression of both genes in retinal cells in a dose-dependent manner. The over-expression was transient, being switched off when cadmium was removed. The involvement of RBBP6 and CRYL1 in the onset of cadmium-induced morphological alterations in adult zebrafish retina is discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping