PUBLICATION
?2 and ?1AP-1 complexes: Different essential functions and regulatory mechanisms in clathrin-dependent protein sorting
- Authors
- Zizioli, D., Geumann, C., Kratzke, M., Mishra, R., Borsani, G., Finazzi, D., Candiello, E., Schu, P.
- ID
- ZDB-PUB-170405-7
- Date
- 2017
- Source
- European journal of cell biology 96(4): 356-368 (Journal)
- Registered Authors
- Borsani, Giuseppe
- Keywords
- AP-1, Clathrin, Vesicular transport, ?1, ?2
- MeSH Terms
-
- Protein Transport/genetics
- Mice
- Clathrin/genetics
- Clathrin/metabolism*
- Zebrafish
- Brain/growth & development
- Brain/metabolism*
- Adaptor Protein Complex gamma Subunits/chemistry
- Adaptor Protein Complex gamma Subunits/genetics
- Adaptor Protein Complex gamma Subunits/metabolism*
- Blood Vessels/growth & development
- Blood Vessels/metabolism
- Signal Transduction
- Protein Multimerization
- Mice, Knockout
- Animals
- Endosomes/metabolism
- Endosomes/ultrastructure
- trans-Golgi Network/metabolism*
- trans-Golgi Network/ultrastructure
- Protein Isoforms/chemistry
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Adipocytes/cytology
- Adipocytes/metabolism
- Models, Molecular
- Adaptor Protein Complex sigma Subunits/chemistry
- Adaptor Protein Complex sigma Subunits/genetics
- Adaptor Protein Complex sigma Subunits/metabolism*
- Protein Structure, Secondary
- Cell Line
- Gene Expression Regulation, Developmental
- Embryo, Mammalian
- Embryo, Nonmammalian
- Fibroblasts/cytology
- Fibroblasts/metabolism
- Transport Vesicles/metabolism*
- Transport Vesicles/ultrastructure
- PubMed
- 28372831 Full text @ Eur. J. Cell Biol.
Citation
Zizioli, D., Geumann, C., Kratzke, M., Mishra, R., Borsani, G., Finazzi, D., Candiello, E., Schu, P. (2017) ?2 and ?1AP-1 complexes: Different essential functions and regulatory mechanisms in clathrin-dependent protein sorting. European journal of cell biology. 96(4):356-368.
Abstract
?2 adaptin is homologous to ?1, but is only expressed in vertebrates while ?1 is found in all eukaryotes. We know little about ?2 functions and their relation to ?1. ?1 is an adaptin of the heterotetrameric AP-1 complexes, which sort proteins in and do form clathrin-coated transport vesicles and they also regulate maturation of early endosomes. ?1 knockout mice develop only to blastocysts and thus ?2 does not compensate ?1-deficiency in development. ?2 has not been classified as a clathrin-coated vesicle adaptor protein in proteome analyses and functions for monomeric ?2 in endosomal protein sorting have been proposed, but adaptin interaction studies suggested formation of heterotetrameric AP-1/?2 complexes. We detected ?2 at the trans-Golgi network, on peripheral vesicles and identified ?2 clathrin-coated vesicles in mice. Ubiquitous ?1A and tissue-specific ?1B adaptins bind ?2 and ?1. ?1B knockout in mice does not effect ?1/?1A AP-1 levels, but ?2/?1A AP-1 levels are increased in brain and adipocytes. Also ?2 is essential in development. In zebrafish AP-1/?2 and AP-1/?1 fulfill different, essential functions in brain and the vascular system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping