PUBLICATION
Muscarinic receptors mediate the endocrine-disrupting effects of an organophosphorus insecticide in zebrafish
- Authors
- Santos da Rosa, J.G., Alcântara Barcellos, H.H., Fagundes, M., Variani, C., Rossini, M., Kalichak, F., Koakoski, G., Acosta Oliveira, T., Idalencio, R., Frandoloso, R., Piato, A.L., José Gil Barcellos, L.
- ID
- ZDB-PUB-170404-5
- Date
- 2017
- Source
- Environmental toxicology 32(7): 1964-1972 (Journal)
- Registered Authors
- Keywords
- Danio rerio, acetylcholine receptors, methyl-parathion, scopolamine, steroidogenesis
- MeSH Terms
-
- Phosphoproteins/genetics
- Phosphoproteins/metabolism
- Water Pollutants, Chemical/toxicity*
- Animals
- Insecticides/toxicity*
- RNA, Messenger/metabolism
- Locomotion/drug effects
- Hydrocortisone/metabolism
- HSP72 Heat-Shock Proteins/genetics
- HSP72 Heat-Shock Proteins/metabolism
- Scopolamine/pharmacology
- Muscarinic Antagonists/pharmacology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Proto-Oncogene Proteins c-fos/metabolism
- Pro-Opiomelanocortin/genetics
- Pro-Opiomelanocortin/metabolism
- Endocrine Disruptors/toxicity*
- Methyl Parathion/toxicity*
- Receptors, Muscarinic/metabolism*
- Zebrafish/metabolism*
- PubMed
- 28371364 Full text @ Env. Tox.
Citation
Santos da Rosa, J.G., Alcântara Barcellos, H.H., Fagundes, M., Variani, C., Rossini, M., Kalichak, F., Koakoski, G., Acosta Oliveira, T., Idalencio, R., Frandoloso, R., Piato, A.L., José Gil Barcellos, L. (2017) Muscarinic receptors mediate the endocrine-disrupting effects of an organophosphorus insecticide in zebrafish. Environmental toxicology. 32(7):1964-1972.
Abstract
The glucocorticoid cortisol, the end product of hypothalamus-pituitary-interrenal axis in zebrafish (Danio rerio), is synthesized via steroidogenesis and promotes important physiological regulations in response to a stressor. The failure of this axis leads to inability to cope with environmental challenges preventing adaptive processes in order to restore homeostasis. Pesticides and agrichemicals are widely used, and may constitute an important class of environmental pollutants when reach aquatic ecosystems and nontarget species. These chemical compounds may disrupt hypothalamus-pituitary-interrenal axis by altering synthesis, structure or function of its constituents. We present evidence that organophosphorus exposure disrupts stress response by altering the expression of key genes of the neural steroidogenesis, causing downregulation of star, hsp70, and pomc genes. This appears to be mediated via muscarinic receptors, since the muscarinic antagonist scopolamine blocked these effects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping