PUBLICATION
Using Morpholinos to Examine Gene Function During Fin Regeneration
- Authors
- Thummel, R., Kathryn Iovine, M.
- ID
- ZDB-PUB-170402-3
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1565: 79-85 (Chapter)
- Registered Authors
- Thummel, Ryan
- Keywords
- Electroporation, Fin regeneration, Gene knockdown, Zebrafish
- MeSH Terms
-
- Animal Fins/growth & development*
- Animals
- Electroporation/methods
- Gene Expression Regulation*
- Gene Knockdown Techniques
- Microinjections
- Morpholinos/administration & dosage
- Morpholinos/genetics*
- Phenotype
- Regeneration/genetics*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 28364235 Full text @ Meth. Mol. Biol.
Citation
Thummel, R., Kathryn Iovine, M. (2017) Using Morpholinos to Examine Gene Function During Fin Regeneration. Methods in molecular biology (Clifton, N.J.). 1565:79-85.
Abstract
In the zebrafish regenerating fin, specific gene-targeting morpholinos have been widely utilized to assess gene function. Unlike in embryos, injection of standard morpholinos in the adult regenerating fin is not sufficient for cellular uptake. Rather, morpholinos are first injected extracellularly into the blastemal compartment, followed by electroporation for cellular uptake. Knockdown phenotypes are evaluated 1-4 days post electroporation. This chapter provides a description of the reagents, equipment, and procedure for successful injection and electroporation of morpholinos into the regenerating fin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping