PUBLICATION
Mind Bomb-Binding Partner RanBP9 Plays a Contributory Role in Retinal Development
- Authors
- Yoo, K.W., Thiruvarangan, M., Jeong, Y.M., Lee, M.S., Maddirevula, S., Rhee, M., Bae, Y.K., Kim, H.G., Kim, C.H.
- ID
- ZDB-PUB-170401-11
- Date
- 2017
- Source
- Molecules and cells 40(4): 271-279 (Journal)
- Registered Authors
- Bae, Young Ki, Kim, Cheol-Hee, Lee, Mi-Sun, Yoo, Kyeong-Won
- Keywords
- mind bomb, notch, RanBP9, retinogenesis, zebrafish
- MeSH Terms
-
- Ubiquitin-Protein Ligases/genetics
- Ubiquitin-Protein Ligases/metabolism*
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- COS Cells
- Animals
- Neurogenesis/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Brain/cytology
- Brain/embryology
- Brain/metabolism
- Down-Regulation
- Gene Knockdown Techniques
- Two-Hybrid System Techniques
- Cyclin-Dependent Kinase Inhibitor p57/genetics
- Cyclin-Dependent Kinase Inhibitor p57/metabolism
- Cell Proliferation
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Retinal Dysplasia/genetics
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism*
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- Ependymoglial Cells/physiology
- Chlorocebus aethiops
- Retina/cytology
- Retina/embryology*
- Retina/metabolism
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/metabolism*
- ELAV-Like Protein 3/genetics
- ELAV-Like Protein 3/metabolism
- PubMed
- 28359144 Full text @ Mol. Cells
Citation
Yoo, K.W., Thiruvarangan, M., Jeong, Y.M., Lee, M.S., Maddirevula, S., Rhee, M., Bae, Y.K., Kim, H.G., Kim, C.H. (2017) Mind Bomb-Binding Partner RanBP9 Plays a Contributory Role in Retinal Development. Molecules and cells. 40(4):271-279.
Abstract
Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of ranbp9 resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker huC, elevation of neural proliferation marker her4, and alteration of cell cycle marker p57kip2. Expression of the Müller glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bomb-binding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping