PUBLICATION
Reproductive toxicity of ?-diketone antibiotic mixtures to zebrafish (Danio rerio)
- Authors
- Wang, X., Ma, Y., Liu, J., Yin, X., Zhang, Z., Wang, C., Li, Y., Wang, H.
- ID
- ZDB-PUB-170328-8
- Date
- 2017
- Source
- Ecotoxicology and environmental safety 141: 160-170 (Journal)
- Registered Authors
- Keywords
- Biomarker, Diketone antibiotics, Histopathological change, Reproductive toxicity, Transcriptome analysis, Zebrafish
- MeSH Terms
-
- Transcriptome/drug effects
- Fluoroquinolones/chemistry
- Fluoroquinolones/toxicity*
- Animals
- Gene Expression Profiling
- Water Pollutants, Chemical/chemistry
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Molecular Sequence Annotation
- Anti-Bacterial Agents/chemistry
- Anti-Bacterial Agents/toxicity*
- Tetracyclines/chemistry
- Tetracyclines/toxicity*
- Male
- Female
- Reproduction/drug effects*
- Reproduction/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 28342328 Full text @ Ecotoxicol. Environ. Saf.
Citation
Wang, X., Ma, Y., Liu, J., Yin, X., Zhang, Z., Wang, C., Li, Y., Wang, H. (2017) Reproductive toxicity of ?-diketone antibiotic mixtures to zebrafish (Danio rerio). Ecotoxicology and environmental safety. 141:160-170.
Abstract
So far, few data are available on the reproductive toxicological assessment of β-diketone antibiotics (DKAs), a class of ubiquitous pseudo-persistent pollutant, in zebrafish (Danio rerio). Herein, we reported the reproductive effects of DKAs by means of transcriptome analysis (F1-zebrafish), changes in a series of reproductive indices (F0-zebrafish) and histopathological observations. A total of 1170, 983 and 1399 genes were found to be differentially expressed when compared control vs. 6.25mg/L, control vs. 12.5mg/L and 6.25 vs. 12.5mg/L DKA-exposure treatments, respectively. Among three comparison groups, 670, 569 and 821 genes were respectively assigned for GO analyses based on matches with sequences of known functions. In 149 KEGG-noted metabolic pathways, the preferential one was the MAPK (mitogen-activated protein kinase) signaling pathway, followed by oxidative phosphorylation, neuroactive ligand-receptor interaction and so on. By qPCR verification, 6 genes (c6ast4, igfbp1b, mrpl42, tnnc2, emc4 and ddit4) showed consistent gene expression with those identified by transcriptome sequencing. Due to DKA-exposure, the concentrations of plasma estradiol and testosterone, and the gonado-somatic index were significantly dose-dependently declined. Also, DKA-exposure led to declining in zebrafish reproductive capacity, reflecting in fertilization, hatchability and egg production. Histopathological observations demonstrated that zebrafish ovary and testis suffered serious damage after DKA-exposure. The 4-oxo-TEMP signals increased obviously with increasing DKA-exposed concentrations, implying disruption of balance between generation and clearance of 1O2. In summary, DKAs not only produce reproductive toxicological effects on F0-zebrafish, but also result in adverse consequences for growth and development of F1-zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping