PUBLICATION
Design and evaluation of an Edwardsiella tarda DNA vaccine co-encoding antigenic and adjuvant peptide
- Authors
- Liu, X., Xu, J., Zhang, H., Liu, Q., Xiao, J., Zhang, Y.
- ID
- ZDB-PUB-170323-26
- Date
- 2016
- Source
- Fish & shellfish immunology 59: 189-195 (Journal)
- Registered Authors
- Liu, Qin
- Keywords
- Bicistronic expression system, Co-encoding, DNA vaccine, Edwardsiella tarda
- MeSH Terms
-
- Adjuvants, Immunologic/pharmacology
- Animals
- Antigens, Bacterial/pharmacology
- Bacterial Proteins/pharmacology*
- Bacterial Vaccines/immunology*
- Edwardsiella tarda/immunology*
- Peptides/immunology
- Random Allocation
- Vaccines, DNA/immunology
- Zebrafish
- PubMed
- 27765700 Full text @ Fish Shellfish Immunol.
Citation
Liu, X., Xu, J., Zhang, H., Liu, Q., Xiao, J., Zhang, Y. (2016) Design and evaluation of an Edwardsiella tarda DNA vaccine co-encoding antigenic and adjuvant peptide. Fish & shellfish immunology. 59:189-195.
Abstract
Edwardsiella tarda is associated with edwardsiellosis in cultured fish, resulting in heavy losses in aquaculture. So far, different types of vaccine have been attempted against E. tarda. In this study, an optimized eukaryotic expression plasmid was developed and an optimized DNA vaccine co-encoding antigenic and adjuvant peptide using a bicistronic expression system was designed. As a result, a modified plasmid harbored cytomegalovirus (CMV) promoter attached with R region of long terminal repeat from human T-cell leukemia virus type 1 (CMV/R) and woodchuck hepatitis virus post-transcriptional response element (WPRE) component showed an increased antigenic gene expression compared with unmodified plasmid. Moreover, the designed system based on bicistronic system exhibited a stronger ability to express antigenic gene and the RPS achieved 87.3% compared with plasmid encoding antigentic gene. Finally, immunological analysis showed that the DNA vaccine induced both innate and adaptive immune responses. These results suggest that co-encoding antigenic and adjuvant proteins might be an efficient strategy to develop DNA vaccines in aquaculture in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping