PUBLICATION
Post-transcriptional and translational regulation of mRNA-like long non-coding RNAs by microRNAs in early developmental stages of zebrafish embryos
- Authors
- Lee, K.T., Nam, J.W.
- ID
- ZDB-PUB-170323-24
- Date
- 2017
- Source
- BMB reports 50(4): 226-231 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions
- Animals
- Embryo, Nonmammalian/metabolism*
- Embryonic Development/genetics
- Gene Expression Regulation
- Gene Silencing
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- RNA, Long Noncoding/antagonists & inhibitors
- RNA, Long Noncoding/genetics
- RNA, Long Noncoding/metabolism*
- Transcriptome
- Zebrafish/growth & development
- PubMed
- 28320503 Full text @ BMB Rep.
Citation
Lee, K.T., Nam, J.W. (2017) Post-transcriptional and translational regulation of mRNA-like long non-coding RNAs by microRNAs in early developmental stages of zebrafish embryos. BMB reports. 50(4):226-231.
Abstract
microRNA (miRNA) represses protein-coding gene via seed pairing to 3' untranslated reiongs (UTRs) of mRNA at the post-transcriptional and translational level. Although working models of miRNA-mediated gene silencing have been successfully established using miRNA transfections and knockouts, the regulatory interaction between miRNA and long non-coding RNA (lncRNA) remain unknown. In particular, how mRNA-resembling lncRNAs with 5' cap, 3' poly(A)-tail, or coding features are regulated by miRNA is yet to be examined. We therefore investigated the functional interaction between miRNAs and lncRNAs with/without those features in miRNA-transfected, early zebrafish embryos. We observed that 5' cap and 3' poly(A)-tails in lncRNAs were the greatest determinants of miRNA-mediated silencing of lncRNAs at both the post-transcriptional and translational level. The lncRNAs verified to contain 5' cap, 3' poly(A)-tail, and canonical miRNA target sites were observed to be repressed in the level of both RNA and ribosome-protected fragment, while those with miRNA target sites and without 5' cap and 3; poly(A)-tail were not robustly repressed by miRNA introduction, suggesting a role as a miRNA-decoy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping