PUBLICATION
Molecular physiology of the hypocalcemic action of fibroblast growth factor 23 in zebrafish (Danio rerio)
- Authors
- Lin, C.H., Hu, H.J., Hwang, P.P.
- ID
- ZDB-PUB-170323-18
- Date
- 2017
- Source
- Endocrinology 158(5): 1347-1358 (Journal)
- Registered Authors
- Hwang, Pung Pung
- Keywords
- rna, messenger, zebrafish, fibroblast growth factor 23
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Calcium/metabolism*
- Calcium Signaling/genetics*
- Embryo, Nonmammalian
- Fibroblast Growth Factors/genetics*
- Fibroblast Growth Factors/metabolism
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Glycoproteins/genetics
- Glycoproteins/metabolism
- Hypocalcemia/genetics*
- Hypocalcemia/metabolism
- Receptors, Calcium-Sensing/genetics
- Receptors, Calcium-Sensing/metabolism
- Signal Transduction/genetics
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 28323996 Full text @ Endocrinology
Citation
Lin, C.H., Hu, H.J., Hwang, P.P. (2017) Molecular physiology of the hypocalcemic action of fibroblast growth factor 23 in zebrafish (Danio rerio). Endocrinology. 158(5):1347-1358.
Abstract
Fibroblast growth factor 23 (FGF23), a hormone required for phosphorus metabolism, was recently proposed to act on Ca2+ uptake; however, the available evidences of how FGF23 controls the body fluid Ca2+ homeostasis needs to be further clarified. The use of zebrafish as a model system revealed that FGF23 is specifically expressed in the corpuscles of Stannius (CS), an organ involved in Ca2+ homeostasis in fish, and its expression is stimulated by ambient water with a high Ca2+ level. The overexpression of FGF23 inhibited Ca2+ uptake by downregulating the mRNA expression of epithelium calcium channel (ECaC). Calcium-sensing receptor (CaSR), which senses changes in extracellular Ca2+ levels and modulates calciotropic hormones in organs controlling Ca2+ homeostasis in vertebrates, was found to be co-expressed with FGF23 in the CS. In addition, upregulated expression of FGF23 mRNA was detected in morphants of stanniocalcin 1 (stc1, another hypocalcemic factor synthesized in the CS), and knockdown of CaSR suppressed such upregulation and enhanced Ca2+ uptake. Taken together, our data indicate that FGF23 functions as a hypocalcemic hormone in zebrafish, and that the CaSR/STC1-FGF23 axis is involved in body fluid Ca2+ homeostasis in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping