ZFIN ID: ZDB-PUB-170323-17
Demonstration of a functional Kiss-Kissr system in amphioxus with implications for origin of neuroendocrine regulation
Wang, P., Wang, M., Ji, G., Yang, S., Zhang, S., Liu, Z.
Date: 2017
Source: Endocrinology   158(5): 1461-1473 (Journal)
Registered Authors: Liu, Zhenhui
Keywords: genes, neurons, neurosecretory systems, peptides, reproductive physiological process, rna, messenger, vertebrates, zebrafish, brain, chordata, kiss1 gene, kissing
MeSH Terms:
  • Animals
  • Biological Evolution*
  • Cloning, Molecular
  • Gene Expression Regulation
  • HEK293 Cells
  • Humans
  • Kisspeptins/genetics*
  • Kisspeptins/isolation & purification
  • Kisspeptins/physiology
  • Lancelets/genetics*
  • Lancelets/metabolism
  • Neurosecretory Systems/physiology*
  • Receptors, G-Protein-Coupled/genetics*
  • Receptors, G-Protein-Coupled/isolation & purification
  • Receptors, G-Protein-Coupled/physiology
  • Transfection
  • Zebrafish
  • Zebrafish Proteins/genetics
PubMed: 28324048 Full text @ Endocrinology
Amphioxus belongs to the Cephalochordata, which is the most basal subphylum of the chordates. Despite many studies on the endocrine system of amphioxus, key information about its regulation remains ambiguous. Here we clearly demonstrated the presence of a functional kisspeptin/kisspeptin receptor (Kiss-Kissr) system, which is involved in the regulation of reproduction in amphioxus. Evolutionary analyses revealed large expansion of Kiss and Kissr (gpr54) genes in amphioxus and they might represent the ancestral type of the Kiss/gpr54 genes in chordates. Amphioxus Kiss was obviously expression at cerebral vesicle and Hatschek's pit, whereas amphioxus gpr54 mRNA was abundantly present in nerve cord, ovary and testes. Amphioxus GPR54-Like1 (GPR54L-1) was shown to be located on the cell membrane. The synthetic amphioxus Kiss-Like (KissL) peptides were capable of activating the amphioxus GPR54L-1 with different potencies, hinting the interaction between Kiss and GPR54. Moreover, the expression of amphioxus gpr54 mRNA was significantly decreased during low or high temperature extremes. Importantly, the injection of amphioxus KissL could cause an elevation of zebrafish blood LH level, and induce the expression of amphioxus gpb5, a gene encoding the ancestral type of vertebrate pituitary glycoprotein hormones. Also, the expression levels of BjkissL-2 or Bjgpr54L-1 were down-regulated post spermiation or spawning. Collectively, amphioxus Kiss-Kissr system has a correlation with the regulation of reproduction. Our studies provide insights into the functional roles and evolutionary history of the Kiss-Kissr system, as well as the origin of the vertebrate neuroendocrine axis for controlling reproduction.