PUBLICATION
In vivo identification of potential uranium protein targets in zebrafish ovaries after chronic waterborne exposure
- Authors
- Eb-Levadoux, Y., Frelon, S., Simon, O., Arnaudguilhem, C., Lobinski, R., Mounicou, S.
- ID
- ZDB-PUB-170321-8
- Date
- 2017
- Source
- Metallomics : integrated biometal science 9(5): 525-534 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Chromatography, Gel
- Female
- Fish Proteins/analysis*
- Fish Proteins/metabolism
- Metalloproteins/analysis*
- Metalloproteins/metabolism
- Models, Molecular
- Ovary/drug effects
- Ovary/metabolism*
- Proteomics/methods
- Spectrometry, Mass, Electrospray Ionization
- Uranium/analysis*
- Uranium/metabolism
- Uranium/toxicity
- Water Pollutants, Chemical/analysis*
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity
- Zebrafish
- PubMed
- 28317950 Full text @ Metallomics
Citation
Eb-Levadoux, Y., Frelon, S., Simon, O., Arnaudguilhem, C., Lobinski, R., Mounicou, S. (2017) In vivo identification of potential uranium protein targets in zebrafish ovaries after chronic waterborne exposure. Metallomics : integrated biometal science. 9(5):525-534.
Abstract
Ecotoxicological studies have indicated the reprotoxicity of uranium (U) in zebrafish, but its molecular mechanisms remain unclear. Due to the non-covalent nature of U-protein complexes, canonical proteomics approaches are often not relevant as they usually use denaturating reagents or solvents. In this study, non-denaturating (ND) methods were used to obtain insight into the nature of U potential targets in ovaries of reproduced and non-reproduced zebrafish after 20 days of exposure to an environmentally relevant U concentration (20 μg L-1). After the ND sample preparation, 1-dimensional (SEC) and 2-dimensional (OGE × SEC) separations followed by ICP-sector-field MS measurements (U, P, Fe, Cu, and Zn) enabled the determination of chemical characteristics (MW, pI) of the metal-protein complexes. Phosphorus and U coelution confirmed the affinity of U for P-containing proteins. In addition, 2D separation allowed the discrimination of Fe-metalloproteins as potential U targets. Finally, 20 protein candidates for U complexation were identified after tryptic digestion conditions by LC-ESI FT MS and a database search. Potential U targets were mainly involved in three biological processes: oxidative stress regulation (SOD, GST), cytoskeleton structure (actin) and embryo early development (vtg, initiation factor).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping