PUBLICATION

Proteomic analysis of three gonad types of swamp eel reveals genes differentially expressed during sex reversal.

Authors
Sheng, Y., Zhao, W., Song, Y., Li, Z., Luo, M., Lei, Q., Cheng, H., Zhou, R.
ID
ZDB-PUB-170316-17
Date
2015
Source
Scientific Reports   5: 10176 (Journal)
Registered Authors
Zhou, Rongjia
Keywords
Animal physiology, Gene expression
MeSH Terms
  • Animals
  • Female
  • Gene Expression Regulation*
  • Gonads/metabolism*
  • Male
  • Protein Interaction Mapping
  • Protein Interaction Maps
  • Proteomics*/methods
  • Sex Determination Processes*
  • Smegmamorpha/genetics*
  • Smegmamorpha/metabolism*
PubMed
25985063 Full text @ Sci. Rep.
Abstract
A variety of mechanisms are engaged in sex determination in vertebrates. The teleost fish swamp eel undergoes sex reversal naturally and is an ideal model for vertebrate sexual development. However, the importance of proteome-wide scanning for gonad reversal was not previously determined. We report a 2-D electrophoresis analysis of three gonad types of proteomes during sex reversal. MS/MS analysis revealed a group of differentially expressed proteins during ovary to ovotestis to testis transformation. Cbx3 is up-regulated during gonad reversal and is likely to have a role in spermatogenesis. Rab37 is down-regulated during the reversal and is mainly associated with oogenesis. Both Cbx3 and Rab37 are linked up in a protein network. These datasets in gonadal proteomes provide a new resource for further studies in gonadal development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping