|ZFIN ID: ZDB-PUB-170316-1|
Optical Projection Tomography as a Novel Method to Visualize and Quantitate Whole-Brain Patterns of Cell Proliferation in the Adult Zebrafish Brain
Lindsey, B.W., Kaslin, J.
|Source:||Zebrafish 14(6): 574-577 (Journal)|
|Registered Authors:||Kaslin, Jan|
|Keywords:||brain development, clearing, neurogenesis, optical projection tomography, regeneration, stem cell proliferation, three-dimensional imaging|
|PubMed:||28296621 Full text @ Zebrafish|
Lindsey, B.W., Kaslin, J. (2017) Optical Projection Tomography as a Novel Method to Visualize and Quantitate Whole-Brain Patterns of Cell Proliferation in the Adult Zebrafish Brain. Zebrafish. 14(6):574-577.
ABSTRACTHow distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using FIJI and IMARIS software.