ZFIN ID: ZDB-PUB-170316-1
Optical Projection Tomography as a Novel Method to Visualize and Quantitate Whole-Brain Patterns of Cell Proliferation in the Adult Zebrafish Brain
Lindsey, B.W., Kaslin, J.
Date: 2017
Source: Zebrafish   14(6): 574-577 (Journal)
Registered Authors: Kaslin, Jan
Keywords: brain development, clearing, neurogenesis, optical projection tomography, regeneration, stem cell proliferation, three-dimensional imaging
MeSH Terms:
  • Animals
  • Brain/anatomy & histology*
  • Brain/growth & development
  • Cell Proliferation*
  • Image Processing, Computer-Assisted/methods*
  • Software
  • Tomography, Optical/methods
  • Tomography, Optical/veterinary*
  • Zebrafish/anatomy & histology*
  • Zebrafish/growth & development
PubMed: 28296621 Full text @ Zebrafish
How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using FIJI and IMARIS software.