PUBLICATION
Toxic effects of perfluorinated compounds at human cellular level and on a model vertebrate
- Authors
- Rainieri, S., Conlledo, N., Langerholc, T., Madorran, E., Sala, M., Barranco, A.
- ID
- ZDB-PUB-170314-9
- Date
- 2017
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 104: 14-25 (Journal)
- Registered Authors
- Keywords
- In vitro, In vivo, Mixtures, Oxidative stress, PFCs, Toxicity, Zebrafish
- MeSH Terms
-
- Animals
- Humans
- Zebrafish/growth & development
- Zebrafish/metabolism
- Cell Line
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/toxicity*
- Models, Biological
- Oxidative Stress/drug effects
- Fluorocarbons/analysis
- Fluorocarbons/toxicity*
- Macrophages/drug effects*
- Macrophages/metabolism
- PubMed
- 28286311 Full text @ Food Chem. Toxicol.
Citation
Rainieri, S., Conlledo, N., Langerholc, T., Madorran, E., Sala, M., Barranco, A. (2017) Toxic effects of perfluorinated compounds at human cellular level and on a model vertebrate. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 104:14-25.
Abstract
This work aims at deepening the understanding of the mode of action of some of the most prominent perfluorinated compounds (PFCs) by detecting in a realistic way their effects. To this end, after adjusting the exposure media taking into account the biological model employed and the physico-chemical properties of PFCs, we evaluated the toxic effects of PFOA, PFOS and PFNA in a human macrophage cell line (TLT cells) and in zebrafish embryos. We performed such evaluation on individual compounds and mixtures. Acute toxicity was greater for PFOS in zebrafish; however, it was greater for PFNA in TLT cells. PFNA was also the compound producing the greatest levels of oxidative stress, both in zebrafish and TLT cells. Additionally, in both biological systems, it showed a much stronger effect on mixtures in comparison to the others PFCs tested in this work. Mixture studies in zebrafish showed that acute toxicity depended on the concentration and that the mixture was far more toxic than the individual compounds. This study highlights the importance of studying PFCs in realistic conditions on various biological models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping