PUBLICATION
Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization
- Authors
- McDougall, M., Choi, J., Kim, H.K., Bobe, G., Stevens, J.F., Cadenas, E., Tanguay, R., Traber, M.G.
- ID
- ZDB-PUB-170311-6
- Date
- 2017
- Source
- Data in brief 11: 432-441 (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- Alpha-tocopherol, Choline, Docosahexaenoic acid, Ferroptosis, Glucose
- MeSH Terms
- none
- PubMed
- 28280764 Full text @ Data Brief
Citation
McDougall, M., Choi, J., Kim, H.K., Bobe, G., Stevens, J.F., Cadenas, E., Tanguay, R., Traber, M.G. (2017) Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization. Data in brief. 11:432-441.
Abstract
The data herein is in support of our research article by McDougall et al. (2017) [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E-) or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet), were spawned to obtain E- and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies. Rescues were attempted using micro-injection into the yolksac using VitE (as a phospholipid emulsion containing d6-α-tocopherol at 0 hpf) or D-glucose (in saline at 24 hpf).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping