PUBLICATION
Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations
- Authors
- Gao, X., Yu, T., Xu, G., Guo, G., Liu, X., Hu, X., Wang, X., Liu, Y., Mao, Q., You, C., Zhou, L.
- ID
- ZDB-PUB-170228-3
- Date
- 2017
- Source
- Scientific Reports 7: 43501 (Journal)
- Registered Authors
- Keywords
- Chemotherapy, CNS cancer, Experimental models of disease
- MeSH Terms
-
- Animals
- Antineoplastic Agents/administration & dosage
- Antineoplastic Agents/pharmacology*
- Apoptosis/drug effects
- Biphenyl Compounds/administration & dosage
- Biphenyl Compounds/pharmacology*
- Cell Line, Tumor
- Cell Proliferation/drug effects
- Disease Models, Animal
- Doxorubicin/administration & dosage
- Doxorubicin/pharmacology*
- Drug Carriers*/chemistry
- Drug Liberation
- Drug Synergism
- Humans
- Lignans/administration & dosage
- Lignans/pharmacology*
- Micelles*
- Nanoparticles/chemistry
- Nanoparticles/ultrastructure
- Neovascularization, Pathologic/drug therapy
- Polyesters/chemistry
- Polyethylene Glycols/chemistry
- X-Ray Diffraction
- Xenograft Model Antitumor Assays
- Zebrafish
- PubMed
- 28240249 Full text @ Sci. Rep.
Citation
Gao, X., Yu, T., Xu, G., Guo, G., Liu, X., Hu, X., Wang, X., Liu, Y., Mao, Q., You, C., Zhou, L. (2017) Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations. Scientific Reports. 7:43501.
Abstract
Combination chemotherapy is an important protocol in glioma therapy and honokiol shows synergistic anticancer effects with doxorubicin. In this paper, honokiol (HK) and doxorubicin (Dox) co-loaded Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) nanoparticles were prepared with a assembly method. The particle size (about 34 nm), morphology, X-ray Powder Diffraction (XRD), in vitro release profile, cytotoxicity and cell proliferation effects were studied in detail. The results indicated that honokiol and doxorubicin could be efficiently loaded into MPEG-PCL nanoparticles simultaneously, and could be released from the micelles in an extended period in vitro. In addition, honokiol and doxorubicin loaded in MPEG-PCL nanoparticles could efficiently suppress glioma cell proliferation and induce cell apoptosis in vitro. Furthermore, Dox-HK-MPEG-PCL micelles inhibited glioma growth more significantly than Dox-MPEG-PCL and HK-MPEG-PCL in both nude mice and zebrafish tumor models. Immunohistochemical analysis indicated that DOX-HK-MPEG-PCL micelles improved Dox's anti-tumor effect by enhancing tumor cell apoptosis, suppressing tumor cell proliferation, and inhibiting angiogenesis. Our data suggest that Dox-HK-MPEG-PCL micelles have the potential to be applied clinically in glioma therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping