PUBLICATION
Modafinil decreases anxiety-like behaviour in zebrafish
- Authors
- Johnson, A., Hamilton, T.J.
- ID
- ZDB-PUB-170224-11
- Date
- 2017
- Source
- PeerJ 5: e2994 (Journal)
- Registered Authors
- Hamilton, Trevor
- Keywords
- Anxiety, Ethanol, Locomotor activity, Modafinil, Novel approach test, Zebrafish
- MeSH Terms
- none
- PubMed
- 28229024 Full text @ Peer J.
Citation
Johnson, A., Hamilton, T.J. (2017) Modafinil decreases anxiety-like behaviour in zebrafish. PeerJ. 5:e2994.
Abstract
Modafinil (2-((diphenylmethyl)sulfinyl)acetamide), a selective dopamine and norepinephrine transporter inhibitor, is most commonly prescribed for narcolepsy but has gained recent interest for treating a variety of disorders. Zebrafish (Danio rerio) are becoming a model of choice for pharmacological and behavioural research. To investigate the behavioural effects of modafinil on anxiety, we administered doses of 0, 2, 20, and 200 mg/L for 30 minutes then tested zebrafish in the novel approach test. In this test, the fish was placed into a circular arena with a novel object in the center and motion-tracking software was used to quantify the time the fish spent in the outer area of the arena (thigmotaxis zone), middle third of the arena (transition zone) and center of the arena, as well as total distance traveled, immobility and meandering. Modafinil caused a decrease in time spent in the thigmotaxis zone and increased time spent in the transition zone across all doses. Modafinil did not significantly alter the time spent in the center zone (near the novel object), the distance moved, meandering, or the duration of time spent immobile. We also validated this test as a measure of anxiety with the administration of ethanol (1%) which decreased time spent in the thigmotaxis zone and increased time spent in the transition zone. These results suggest that modafinil decreases anxiety-like behaviour in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping