PUBLICATION
Molecular Engineering and Design of Semiconducting Polymer Dots with Narrow-Band, Near-Infrared Emission for In Vivo Biological Imaging
- Authors
- Ke, C.S., Fang, C.C., Yan, J.Y., Tseng, P.J., Pyle, J.R., Chen, C.P., Lin, S.Y., Chen, J., Zhang, X., Chan, Y.H.
- ID
- ZDB-PUB-170222-4
- Date
- 2017
- Source
- ACS nano 11(3): 3166-3177 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Drug Design*
- Female
- Humans
- Infrared Rays
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Microscopy, Fluorescence
- Molecular Structure
- Neoplasms, Experimental/diagnostic imaging
- Optical Imaging*
- Polymers/chemical synthesis
- Polymers/chemistry*
- Quantum Dots/chemistry*
- Semiconductors
- Zebrafish/embryology
- PubMed
- 28221751 Full text @ ACS Nano
Citation
Ke, C.S., Fang, C.C., Yan, J.Y., Tseng, P.J., Pyle, J.R., Chen, C.P., Lin, S.Y., Chen, J., Zhang, X., Chan, Y.H. (2017) Molecular Engineering and Design of Semiconducting Polymer Dots with Narrow-Band, Near-Infrared Emission for In Vivo Biological Imaging. ACS nano. 11(3):3166-3177.
Abstract
This article describes the design and synthesis of donor-bridge-acceptor based semiconducting polymer dots (Pdots) that exhibit narrow-band emissions, ultrahigh brightness, and large Stokes shifts in the near-infrared (NIR) region. We systematically investigated the effect of π-bridges on the fluorescence quantum yields of the donor-bridge-acceptor based Pdots. The Pdots could be excited by a 488-nm or 532-nm laser and have a high fluorescence quantum yield of 33% with a Stokes shift over 200 nm. The emission full width at half-maximum of the Pdots can be as narrow as 29 nm, about 2.5 times narrower than that of inorganic quantum dots at the same emission wavelength region. The average per-particle brightness of the Pdots is at least 3 times larger than that of the commercially available quantum dots. The excellent biocompatibility of these Pdots was demonstrated in vivo and their specific cellular labeling capability was also approved by different cell lines. By taking advantage of the durable brightness and remarkable stability of these NIR fluorescent Pdots, we performed in vivo microangiography imaging on living zebrafish embryos and long-term tumor monitoring on mice. We anticipate these donor-bridge-acceptor based NIR-fluorescent Pdots with narrow-band emissions to find broad use in a variety of multiplexed biological applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping