PUBLICATION
Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone
- Authors
- Shi, W.J., Ying, G.G., Huang, G.Y., Liang, Y.Q., Hu, L.X., Zhao, J.L., Zhang, J.N.
- ID
- ZDB-PUB-170220-1
- Date
- 2017
- Source
- Bulletin of environmental contamination and toxicology 99(1): 39-45 (Journal)
- Registered Authors
- Keywords
- Biochemical alteration, Dydrogesterone, Transcription, Zebrafish
- MeSH Terms
-
- Animals
- Dydrogesterone/toxicity*
- Eleutherococcus
- Embryo, Nonmammalian/drug effects*
- Endocrine Disruptors/toxicity*
- Endocrine System
- Progesterone Congeners
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 28214940 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Shi, W.J., Ying, G.G., Huang, G.Y., Liang, Y.Q., Hu, L.X., Zhao, J.L., Zhang, J.N. (2017) Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone. Bulletin of environmental contamination and toxicology. 99(1):39-45.
Abstract
Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144 h exposure to DDG. The results of qPCR analysis showed that DDG exposure significantly suppressed the transcriptions of target genes involved in hypothalamic-pituitary-thyroid (HPT) axis, while it induced the expression of target genes mRNA belonging to hypothalamic-pituitary-gonad (HPG) axis. In addition, ATR-FTIR spectroscopy analysis showed that the biochemical alterations of protein, nucleic acid and lipid were observed following DDG treatment. The finding from this study suggests that DDG exposure could have potential multiple effects in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping