PUBLICATION
Th17 master transcription factors ROR? and ROR? regulate the expression of IL-17C, IL-17D and IL-17F in Cynoglossus semilaevis
- Authors
- Chi, H., Bøgwald, J., Dalmo, R.A., Zhang, W., Hu, Y.H.
- ID
- ZDB-PUB-170214-83
- Date
- 2016
- Source
- Developmental and comparative immunology 55: 169-78 (Journal)
- Registered Authors
- Keywords
- Cynoglossus semilaevis, IL-17, Promoter activity, ROR?, ROR?
- MeSH Terms
-
- Cell Line
- Flatfishes/immunology*
- Gene Expression Regulation
- Transgenes/genetics
- Receptors, Cytoplasmic and Nuclear/genetics
- Receptors, Cytoplasmic and Nuclear/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Interleukin-27/genetics
- Interleukin-27/metabolism
- Vibrio/immunology*
- DNA Virus Infections/immunology*
- Amino Acid Sequence
- Animals
- Molecular Sequence Data
- Th17 Cells/immunology
- Interleukin-17/genetics
- Interleukin-17/metabolism
- Nuclear Receptor Subfamily 1, Group F, Member 3/genetics
- Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism*
- Vibrio Infections/immunology*
- Iridoviridae/immunology*
- PubMed
- 26547017 Full text @ Dev. Comp. Immunol.
Citation
Chi, H., Bøgwald, J., Dalmo, R.A., Zhang, W., Hu, Y.H. (2016) Th17 master transcription factors ROR? and ROR? regulate the expression of IL-17C, IL-17D and IL-17F in Cynoglossus semilaevis. Developmental and comparative immunology. 55:169-78.
Abstract
The RAR-related orphan receptors (RORs) are members of the nuclear receptor family of intracellular transcription factors. In this study, we examined the regulatory properties of ROR? (CsROR?) and ROR? (CsROR?) in tongue sole (Cynoglossus semilaevis). CsROR? and CsROR? expression was detected in major lymphoid organs and altered to significant extents after bacterial and viral infection. CsROR? enhanced the activities of CsIL-17C, CsIL-17D, and CsIL-17F promoters, which contain CsROR? and CsROR? binding sites. CsROR? also upregulated the promoter activities of CsIL-17D and CsIL-17F but not CsIL-17C. CsROR? and CsROR? proteins were detected in the nucleus, and overexpression of CsROR? in tongue sole significantly increased the expression of CsIL-17C, CsIL-17D, and CsIL-17F, whereas overexpression of CsROR? significantly increased the expression of CsIL-17C and CsIL-17F but no CsIL-17D. These results indicate that ROR? and ROR? in teleost regulate the expression of IL-17 members in different manners.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping