PUBLICATION
Regulatory circuit rewiring and functional divergence of the duplicate admp genes in dorsoventral axial patterning
- Authors
- Chang, Y.C., Pai, C.Y., Chen, Y.C., Ting, H.C., Martinez, P., Telford, M.J., Yu, J.K., Su, Y.H.
- ID
- ZDB-PUB-170214-55
- Date
- 2016
- Source
- Developmental Biology 410: 108-18 (Journal)
- Registered Authors
- Keywords
- Acorn worm, Admp, Ambulacraria, BMP, Sea urchin
- MeSH Terms
-
- Animals
- Body Patterning*
- Bone Morphogenetic Proteins/genetics
- Bone Morphogenetic Proteins/physiology*
- Gene Duplication*
- Phylogeny
- Sea Urchins/embryology
- Signal Transduction
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/physiology*
- Zebrafish/embryology
- PubMed
- 26719126 Full text @ Dev. Biol.
Citation
Chang, Y.C., Pai, C.Y., Chen, Y.C., Ting, H.C., Martinez, P., Telford, M.J., Yu, J.K., Su, Y.H. (2016) Regulatory circuit rewiring and functional divergence of the duplicate admp genes in dorsoventral axial patterning. Developmental Biology. 410:108-18.
Abstract
The spatially opposed expression of Antidorsalizing morphogenetic protein (Admp) and BMP signals controls dorsoventral (DV) polarity across Bilateria and hence represents an ancient regulatory circuit. Here, we show that in addition to the conserved admp1 that constitutes the ancient circuit, a second admp gene (admp2) is present in Ambulacraria (Echinodermata+Hemichordata) and two marine worms belonging to Xenoturbellida and Acoelomorpha. The phylogenetic distribution implies that the two admp genes were duplicated in the Bilaterian common ancestor and admp2 was subsequently lost in chordates and protostomes. We show that the ambulacrarian admp1 and admp2 are under opposite transcriptional control by BMP signals and knockdown of Admps in sea urchins impaired their DV polarity. Over-expression of either Admps reinforced BMP signaling but resulted in different phenotypes in the sea urchin embryo. Our study provides an excellent example of signaling circuit rewiring and protein functional changes after gene duplications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping