ZFIN ID: ZDB-PUB-170214-261
AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation
Cianfanelli, V., Fuoco, C., Lorente, M., Salazar, M., Quondamatteo, F., Gherardini, P.F., De Zio, D., Nazio, F., Antonioli, M., D'Orazio, M., Skobo, T., Bordi, M., Rohde, M., Dalla Valle, L., Helmer-Citterich, M., Gretzmeier, C., Dengjel, J., Fimia, G.M., Piacentini, M., Di Bartolomeo, S., Velasco, G., Cecconi, F.
Date: 2015
Source: Nature cell biology   17: 20-30 (Journal)
Registered Authors: Dalla Valle, Luisa, Piacentini, Mauro, Skobo, Tatjana
Keywords: Cell proliferation, Cell signalling, Macroautophagy
MeSH Terms:
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/physiology*
  • Animals
  • Autophagy/genetics*
  • Cell Division/genetics
  • Cell Line, Tumor
  • Cell Transformation, Neoplastic/genetics*
  • Female
  • Genes, Tumor Suppressor/physiology*
  • HEK293 Cells
  • Haploinsufficiency*
  • HeLa Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Phosphorylation
  • Protein Phosphatase 2/metabolism
  • Proto-Oncogene Proteins c-myc/metabolism*
  • RNA Interference
  • RNA, Small Interfering
  • TOR Serine-Threonine Kinases/antagonists & inhibitors*
  • Zebrafish
PubMed: 25438055 Full text @ Nat. Cell Biol.
ABSTRACT
Inhibition of a main regulator of cell metabolism, the protein kinase mTOR, induces autophagy and inhibits cell proliferation. However, the molecular pathways involved in the cross-talk between these two mTOR-dependent cell processes are largely unknown. Here we show that the scaffold protein AMBRA1, a member of the autophagy signalling network and a downstream target of mTOR, regulates cell proliferation by facilitating the dephosphorylation and degradation of the proto-oncogene c-Myc. We found that AMBRA1 favours the interaction between c-Myc and its phosphatase PP2A and that, when mTOR is inhibited, it enhances PP2A activity on this specific target, thereby reducing the cell division rate. As expected, such a de-regulation of c-Myc correlates with increased tumorigenesis in AMBRA1-defective systems, thus supporting a role for AMBRA1 as a haploinsufficient tumour suppressor gene.
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