PUBLICATION
Aldehyde dehydrogenase homologous folate enzymes: Evolutionary switch between cytoplasmic and mitochondrial localization
- Authors
- Krupenko, N.I., Holmes, R.S., Tsybovsky, Y., Krupenko, S.A.
- ID
- ZDB-PUB-170214-235
- Date
- 2015
- Source
- Chemico-biological interactions 234: 12-7 (Journal)
- Registered Authors
- Keywords
- ALDH1L enzymes, Aldehyde dehydrogenases, Enzyme mechanism, Evolution, Folate metabolism, Mitochondria
- MeSH Terms
-
- Aldehyde Dehydrogenase/metabolism*
- Amino Acid Sequence
- Animals
- Cytoplasm/enzymology*
- Cytoplasm/metabolism*
- Evolution, Molecular
- Folic Acid/metabolism*
- Humans
- Invertebrates/enzymology
- Invertebrates/metabolism
- Mitochondria/enzymology*
- Mitochondria/metabolism*
- Phylogeny
- Vertebrates/embryology
- Vertebrates/metabolism
- Zebrafish/metabolism
- PubMed
- 25549576 Full text @ Chem. Biol. Interact.
Citation
Krupenko, N.I., Holmes, R.S., Tsybovsky, Y., Krupenko, S.A. (2015) Aldehyde dehydrogenase homologous folate enzymes: Evolutionary switch between cytoplasmic and mitochondrial localization. Chemico-biological interactions. 234:12-7.
Abstract
Cytosolic and mitochondrial 10-formyltetrahydrofolate dehydrogenases are products of separate genes in vertebrates but only one such gene is present in invertebrates. There is a significant degree of sequence similarity between the two enzymes due to an apparent origin of the gene for the mitochondrial enzyme (ALDH1L2) from the duplication of the gene for the cytosolic enzyme (ALDH1L1). The primordial ALDH1L gene originated from a natural fusion of three unrelated genes, one of which was an aldehyde dehydrogenase. Such structural organization defined the catalytic mechanism of these enzymes, which is similar to that of aldehyde dehydrogenases. Here we report the analysis of ALDH1L1 and ALDH1L2 genes from different species and their phylogeny and evolution. We also performed sequence and structure comparison of ALDH1L enzymes possessing aldehyde dehydrogenase catalysis to those lacking this feature in an attempt to explain mechanistic differences between cytoplasmic ALDH1L1 and mitochondrial ALDH1L2 enzymes and to better understand their functional roles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping