ZFIN ID: ZDB-PUB-170206-13
Spatiotemporal expression of foxo4, foxo6a, and foxo6b in the developing brain and retina are transcriptionally regulated by PI3K signaling in zebrafish
Lin, S.J., Chiang, M.C., Shih, H.Y., Chiang, K.C., Cheng, Y.C.
Date: 2017
Source: Development genes and evolution   227(3): 219-230 (Journal)
Registered Authors:
Keywords: Brain, Foxo, Neural development, PI3K, Retina
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Brain/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Forkhead Transcription Factors/genetics*
  • Gene Expression Regulation
  • Phosphatidylinositol 3-Kinases/antagonists & inhibitors
  • Phylogeny
  • Retina/metabolism*
  • Sequence Alignment
  • Signal Transduction
  • Transcription, Genetic
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
PubMed: 28154937 Full text @ Dev. Genes Evol.
The forkhead box subclass O (FoxO) family of proteins is a group of highly evolutionary conserved transcription factors that regulate various cellular processes and embryonic development. Dysregulated expressions of FOXO genes have been identified in numerous tumors and genetic disorders. The expression of FOXO/Foxo, particularly FOXO4/Foxo4 and FOXO6/Foxo6, in the developing nervous system has not been fully characterized. Here, we identified zebrafish foxo4, foxo6a, and foxo6b homologs and demonstrated that all three genes were expressed in the developing nervous system. foxo4, foxo6a, and foxo6b displayed ubiquitous expression in the brain and later in distinct brain tissues. In addition, these three genes were expressed in different retinal layers in a time-dependent manner. Furthermore, the mRNA expression of all three genes was significantly downregulated after treatment with a selective PI3-kinase (PI3K) inhibitor, LY294002. Our results suggest that foxo4, foxo6a, and foxo6b play important roles in the developing brain and retina and that the transcriptional levels of these genes are regulated by PI3-kinase signaling.