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ZFIN ID: ZDB-PUB-170204-1
Microstructured Surface Arrays for Injection of Zebrafish Larvae
Ellett, F., Irimia, D.
Date: 2017
Source: Zebrafish   14(2): 140-145 (Journal)
Registered Authors: Ellett, Felix
Keywords: infection, larvae, microinjection, microstructures, xenotransplantation
MeSH Terms:
  • Animals
  • Laboratory Animal Science
  • Larva
  • Microinjections/methods
  • Microinjections/veterinary*
  • Silicones
  • Zebrafish/anatomy & histology*
PubMed: 28151697 Full text @ Zebrafish
ABSTRACT
Microinjection of zebrafish larvae is an essential technique for delivery of treatments, dyes, microbes, and xenotransplantation into various tissues. Although a number of casts are available to orient embryos at the single-cell stage, no device has been specifically designed to position hatching-stage larvae for microinjection of different tissues. In this study, we present a reusable silicone device consisting of arrayed microstructures, designed to immobilize 2 days postfertilization larvae in lateral, ventral, and dorsal orientations, while providing maximal access to target sites for microinjection. Injection of rhodamine dextran was used to demonstrate the utility of this device for precise microinjection of multiple anatomical targets.
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