|ZFIN ID: ZDB-PUB-170204-1|
Microstructured Surface Arrays for Injection of Zebrafish Larvae
Ellett, F., Irimia, D.
|Source:||Zebrafish 14(2): 140-145 (Journal)|
|Registered Authors:||Ellett, Felix|
|Keywords:||infection, larvae, microinjection, microstructures, xenotransplantation|
|PubMed:||28151697 Full text @ Zebrafish|
Ellett, F., Irimia, D. (2017) Microstructured Surface Arrays for Injection of Zebrafish Larvae. Zebrafish. 14(2):140-145.
ABSTRACTMicroinjection of zebrafish larvae is an essential technique for delivery of treatments, dyes, microbes, and xenotransplantation into various tissues. Although a number of casts are available to orient embryos at the single-cell stage, no device has been specifically designed to position hatching-stage larvae for microinjection of different tissues. In this study, we present a reusable silicone device consisting of arrayed microstructures, designed to immobilize 2 days postfertilization larvae in lateral, ventral, and dorsal orientations, while providing maximal access to target sites for microinjection. Injection of rhodamine dextran was used to demonstrate the utility of this device for precise microinjection of multiple anatomical targets.