ZFIN ID: ZDB-PUB-170129-25
Studying the adaptive immune system in zebrafish by transplantation of hematopoietic precursor cells
Iwanami, N., Hess, I., Schorpp, M., Boehm, T.
Date: 2017
Source: Methods in cell biology   138: 151-161 (Chapter)
Registered Authors: Boehm, Tom, Hess, Isabell, Schorpp, Michael
Keywords: Hematopoiesis, Thymus, Transplantation, cmyb
MeSH Terms:
  • Adaptive Immunity/genetics
  • Adaptive Immunity/immunology*
  • Animals
  • Embryo, Nonmammalian
  • Hematopoiesis/genetics
  • Hematopoietic Stem Cell Transplantation/methods*
  • Hematopoietic Stem Cells/immunology
  • Molecular Biology/methods*
  • Mutation
  • Proto-Oncogene Proteins c-myb/genetics
  • Proto-Oncogene Proteins c-myb/immunology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed: 28129842 Full text @ Meth. Cell. Biol.
Traditionally, transplantation has been a major experimental procedure to study the development and function of hematopoietic and immune systems. Here, we describe the use of a zebrafish strain lacking definitive hematopoiesis (cmybI181N) for interspecific analysis of hematopoietic and immune cell development. Without conditioning prior to transplantation, allogeneic and xenogeneic hematopoietic progenitor cells stably engraft in adult zebrafish homozygous for the cmyb mutation. This unique animal model can be used to genetically and functionally disentangle universal and species-specific contributions of the microenvironment to hematopoietic progenitor cell maintenance and development.