PUBLICATION

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes

Authors
Ribas, L., Liew, W.C., Díaz, N., Sreenivasan, R., Orbán, L., Piferrer, F.
ID
ZDB-PUB-170125-9
Date
2017
Source
Proceedings of the National Academy of Sciences of the United States of America   114(6): E941-E950 (Journal)
Registered Authors
Liew, Woei Chang, Sreenivasan, Rajini
Keywords
global warming, masculinization, polygenic sex determination, temperature, zebrafish
Datasets
GEO:GSE51434
MeSH Terms
  • Animals
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Ontology
  • Gonads/embryology
  • Gonads/growth & development
  • Gonads/metabolism*
  • Hot Temperature*
  • Male
  • Models, Animal
  • Sex Differentiation/genetics*
  • Sex Ratio
  • Temperature
  • Testis/embryology
  • Testis/growth & development
  • Testis/metabolism
  • Transcriptome*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed
28115725 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Understanding environmental influences on sex ratios is important for the study of the evolution of sex-determining mechanisms and for evaluating the effects of global warming and chemical pollution. Fishes exhibit sexual plasticity, but the underlying mechanisms of environmental effects on their reproduction are unclear even in the well-established teleost research model, the zebrafish. Here we established the conditions to study the effects of elevated temperature on zebrafish sex. We showed that sex ratio response to elevated temperature is family-specific and typically leads to masculinization (female-to-male sex reversal), resulting in neomales. These results uncovered genotype-by-environment interactions that support a polygenic sex determination system in domesticated (laboratory) zebrafish. We found that some heat-treated fish had gene expression profiles similar to untreated controls of the same sex, indicating that they were resistant to thermal effects. Further, most neomales had gonadal transcriptomes similar to that of regular males. Strikingly, we discovered heat-treated females that displayed a normal ovarian phenotype but with a "male-like" gonadal transcriptome. Such major transcriptomic reprogramming with preserved organ structure has never been reported. Juveniles were also found to have a male-like transcriptome shortly after exposure to heat. These findings were validated by analyzing the expression of genes and signaling pathways associated with sex differentiation. Our results revealed a lasting thermal effect on zebrafish gonads, suggesting new avenues for detection of functional consequences of elevated temperature in natural fish populations in a global warming scenario.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes