ZFIN ID: ZDB-PUB-170110-2
Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging
Cutrale, F., Trivedi, V., Trinh, L.A., Chiu, C.L., Choi, J.M., Artiga, M.S., Fraser, S.E.
Date: 2017
Source: Nature Methods   14(2): 149-152 (Journal)
Registered Authors: Fraser, Scott E., Trinh, Le
Keywords: Developmental biology, Fluorescence imaging, Image processing
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Color
  • Embryo, Nonmammalian
  • Fourier Analysis
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Image Processing, Computer-Assisted
  • Software*
  • Time-Lapse Imaging/methods*
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed: 28068315 Full text @ Nat. Methods
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.