PUBLICATION
Time-dependent interacting effects of caffeine, diazepam, and ethanol on zebrafish behaviour
- Authors
- Tran, S., Fulcher, N., Nowicki, M., Desai, P., Tsang, B., Facciol, A., Chow, H., Gerlai, R.
- ID
- ZDB-PUB-161228-9
- Date
- 2017
- Source
- Progress in neuro-psychopharmacology & biological psychiatry 75: 16-27 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Caffeine, Diazepam, Drug interaction, Ethanol, Time-dependent behavioural changes
- MeSH Terms
-
- Analysis of Variance
- Animals
- Behavior, Animal/drug effects*
- Caffeine/pharmacology*
- Central Nervous System Depressants/pharmacology*
- Central Nervous System Stimulants/pharmacology*
- Diazepam/pharmacology*
- Dose-Response Relationship, Drug
- Ethanol/pharmacology*
- Female
- Male
- Time Factors
- Zebrafish/physiology*
- PubMed
- 28025019 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
Citation
Tran, S., Fulcher, N., Nowicki, M., Desai, P., Tsang, B., Facciol, A., Chow, H., Gerlai, R. (2017) Time-dependent interacting effects of caffeine, diazepam, and ethanol on zebrafish behaviour. Progress in neuro-psychopharmacology & biological psychiatry. 75:16-27.
Abstract
Zebrafish have become a popular animal model for behavioural pharmacology due to their small size, rapid development, and amenability to high throughput behavioural drug screens. Furthermore, water-soluble compounds can be administered via immersion of the fish in the drug solution, which provides a non-invasive drug delivery method. Numerous studies have demonstrated stimulant effects of alcohol. Diazepam and caffeine, on the other hand have been found to have inhibitory effect on locomotor activity in zebrafish. However, the time-dependent changes induced by these psychoactive drugs are rarely reported, and potential drug interactions have not been examined in zebrafish, despite the translational relevance of this question. In the current study, we examine time- and dose-dependent changes in zebrafish following exposure to caffeine, diazepam, and ethanol quantifying four different behavioural parameters over a 30min recording session. We subsequently analyze potential drug-drug interactions by co-administering the three drugs in different combinations. Our time-course and dose-response analyses for each of the three drugs represent so far the most detailed studies available serving as a foundation for future psychopharmacology experiments with zebrafish. Furthermore, we report significant interactions between the three drugs corroborating findings obtained with rodent models as well as in humans, providing translational relevance for the zebrafish model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping