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ZFIN ID: ZDB-PUB-161224-17
Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR
Chen, L., Hu, Y., He, J., Chen, J., Giesy, J.P., Xie, P.
Date: 2017
Source: Environmental science & technology   51(1): 596-607 (Journal)
Registered Authors: Chen, Jun
Keywords: none
MeSH Terms:
  • Animals
  • Liver/metabolism
  • Male
  • Metabolome
  • Microcystins/toxicity
  • Proteome/metabolism*
  • Proteomics
  • Zebrafish/metabolism*
PubMed: 28005350 Full text @ Env. Sci. Tech.
ABSTRACT
In this study, for the first time, changes in expressions of proteins and profiles of metabolites in liver of the small, freshwater fish [Formula: see text] (zebrafish) were investigated after long-term exposure to environmentally relevant concentrations of microcystin-LR (MC-LR). Male zebrafish were exposed via water to 1 or 10 μg MC-LR/L for 90 days, and iTRAQ-based proteomics and 1H NMR-based metabolomics were employed. Histopathological observations showed that MC-LR caused damage to liver, and the effects were more pronounced in fish exposed to 10 μg MC-LR/L. Metabolomic analysis also showed alterations of hepatic function, which included changes in a number of metabolic pathways, including small molecules involved in energy, glucose, lipids, and amino acids metabolism. Concentrations of lactate were significantly greater in individuals exposed to MC-LR than in unexposed controls. This indicated a shift toward anaerobic metabolism, which was confirmed by impaired respiration in mitochondria. Proteomics revealed that MC-LR significantly influenced multiple proteins, including those involved in folding of proteins and metabolism. Endoplasmic reticulum stress contributed to disturbance of metabolism of lipids in liver of zebrafish exposed to MC-LR. Identification of proteins and metabolites in liver of zebrafish responsive to MC-LR provides insights into mechanisms of chronic toxicity of MCs.
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