PUBLICATION
Regulator of G protein signaling 2 (Rgs2) regulates neural crest development through Ppar?-Sox10 cascade
- Authors
- Lin, S.J., Chiang, M.C., Shih, H.Y., Hsu, L.S., Yeh, T.H., Huang, Y.C., Lin, C.Y., Cheng, Y.C.
- ID
- ZDB-PUB-161217-11
- Date
- 2017
- Source
- Biochimica et biophysica acta 1864(3): 463-474 (Journal)
- Registered Authors
- Lin, Sheng-Jia
- Keywords
- Ppar?, Rgs2, Sox10, neural crest, zebrafish
- MeSH Terms
-
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Neurogenesis/genetics*
- SOXE Transcription Factors/genetics*
- SOXE Transcription Factors/metabolism
- Neural Crest/growth & development
- Neural Crest/metabolism*
- Sequence Homology, Amino Acid
- Wnt Signaling Pathway
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Wnt Proteins/genetics
- Wnt Proteins/metabolism
- Gene Expression Regulation, Developmental
- Sequence Alignment
- RGS Proteins/genetics*
- RGS Proteins/metabolism
- Embryo, Nonmammalian
- Transcriptional Activation
- Amino Acid Sequence
- Promoter Regions, Genetic
- PPAR delta/genetics*
- PPAR delta/metabolism
- Animals
- PubMed
- 27979767 Full text @ Biochim. Biophys. Acta
Citation
Lin, S.J., Chiang, M.C., Shih, H.Y., Hsu, L.S., Yeh, T.H., Huang, Y.C., Lin, C.Y., Cheng, Y.C. (2017) Regulator of G protein signaling 2 (Rgs2) regulates neural crest development through Ppar?-Sox10 cascade. Biochimica et biophysica acta. 1864(3):463-474.
Abstract
Neural crest cells are multipotent progenitors that migrate extensively and differentiate into numerous derivatives. The developmental plasticity and migratory ability of neural crest cells render them an attractive model for studying numerous aspects of cell progression. We observed that zebrafish rgs2 was expressed in neural crest cells. Disrupting Rgs2 expression by using a dominant negative rgs2 construct or rgs2 morpholinos reduced GTPase-activating protein activity, induced the formation of neural crest progenitors, increased the proliferation of nonectomesenchymal neural crest cells, and inhibited the formation of ectomesenchymal neural crest derivatives. The transcription of pparda (which encodes Ppar?, a Wnt-activated transcription factor) was upregulated in Rgs2-deficient embryos, and Ppar? inhibition using a selective antagonist in the Rgs2-deficient embryos repaired neural crest defects. Our results clarify the mechanism through which the Rgs2-Ppar? cascade regulates neural crest development; specifically, Ppar? directly binds to the promoter and upregulates the transcription of the neural crest specifier sox10. This study reveals a unique regulatory mechanism, the Rgs2-Ppar?-Sox10 signaling cascade, and defines a key molecular regulator, Rgs2, in neural crest development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping