PUBLICATION
Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish
- Authors
- Dai, Y., Prithiviraj, N., Gan, J., Zhang, X.A., Yan, J.
- ID
- ZDB-PUB-161216-10
- Date
- 2016
- Source
- Scientific Reports 6: 38693 (Journal)
- Registered Authors
- Yan, Jizhou
- Keywords
- Experimental models of disease, Molecular medicine
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Autophagy/drug effects
- Biocompatible Materials/pharmacology
- Cell Line
- Cell Proliferation/genetics
- Cell Survival/drug effects
- Epidermis/drug effects
- Epidermis/pathology
- Fluorescence
- Gene Expression Regulation/drug effects
- Jaw/pathology*
- Lysosomes/metabolism
- Mitochondria/metabolism
- Models, Biological
- Phenotype
- Proteasome Endopeptidase Complex/metabolism
- Regeneration/drug effects*
- Starfish/anatomy & histology
- Starfish/chemistry*
- Tissue Extracts/pharmacology*
- Wound Healing*
- Zebrafish
- PubMed
- 27974833 Full text @ Sci. Rep.
Citation
Dai, Y., Prithiviraj, N., Gan, J., Zhang, X.A., Yan, J. (2016) Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish. Scientific Reports. 6:38693.
Abstract
Natural bioactive materials provide an excellent pool of molecules for regenerative therapy. In the present study, we amputate portions of the arms of Archaster typicus starfish, extract and separate the active biomaterials, and compare the effects of each fraction on in vitro wound healing and in vivo lower jaw regeneration of zebrafish. Compared with crude extract, normal hexane fractions (NHFs) have a remarkable effect on cellular proliferation and collective migration, and exhibit fibroblast-like morphology, while methanol-water fractions (MWFs) increase cell size, cell-cell adhesion, and cell death. Relative to moderate mitochondrial and lysosomal aggregation in NHFs-cultured cells, MWFs-cultured cells contain more and bigger lysosomal accumulations and clump detachment. The in vivo zebrafish lower jaw regeneration model reveals that NHFs enhance blastema formation and vasculogenesis, while MWFs inhibit fibrogenesis and induce cellular transformation. Gene expression analyses indicate that NHFs and MWFs separately activate blastema-characteristic genes as well as those genes-related to autophagy, proteasome, and apoptosis either during cell scratch healing or ganciclovir-induced apoptosis. Our results suggest that bioactive compounds from NHFs and MWFs could induce blastema formation and remodeling, respectively, and prevent tissue overgrowth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping